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Products >  Cell_Biology >  Cancer_and_Inflammation >  ELISA_Kits_N-Z >  Osteopontin_N-Half

Osteopontin N-Half ELISA Kit

Osteopontin (OPN), a calcium-binding glycosylated phosphoprotein, is secreted by many cell types. Osteopontin is also known as Spp-1, Apl-1, BNSP, bone sialoprotein 1, ETA-1, and minopontin. It is expressed in osteoblasts, macrophages, activated T cells, kidney tubule cells, and vascular smooth muscle cells. Osteopontin is post-translationally glycosylated and phosphorylated; the molecular weight varies within the range of 44-66 kDa, depending on the amount of modification. Thrombin cleaves osteopontin into N-terminal and C-terminal fragments. The N-terminal fragment contains an Arg-Gly-Asp (RGD) amino acid motif that is also found in other extracellular proteins that bind members of the integrin family of cell-surface receptors.

These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to the N-terminal fragment of osteopontin protein; one is precoated on the ELISA plate and the other is HRP-conjugated. These assay kits can be used to measure soluble human or mouse osteopontin protein in serum, EDTA plasma, synovial fluid, urine, or cell culture supernatants.

At-A-Glance Documents Images & Data

Features

  • Capable of measuring the N-terminal fragment of human or mouse osteopontin
  • Sensitivity: 3.09 pmol/L (Cat.# 27258); 5.63 pmol/L (Cat.# 27259)

Applications

  • Measuring the N-terminal fragment of human or mouse osteopontin in serum, EDTA plasma, synovial fluid, urine, or cell culture supernatants

Components

Precoated plate: Anti-OPN Mouse IgG MoAb Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-OPN(1-17) Rabbit
IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant OPN0.5 mL x 2
EIA buffer : 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 6.25–400 pmol/L (Cat.# 27258); 25–1600 pmol/L (Cat.# 27259)
  • Assay sensitivity: 3.09 pmol/L (Cat.# 27258); 5.63 pmol/L (Cat.# 27259)
  • Assay duration: Approximately 3–3.5 hours
  • Test specimen type: EDTA plasma, synovial fluid, urine, or cell culture supernatants (Cat.# 27258); EDTA plasma, urine, or cell culture supernatants (Cat.# 27259)
  • Sample volume per well: 100 µL



 
 
Products
Cat. # Product Package Size Price # of Units Select
27258A Human Osteopontin N-Half Assay Kit - IBL 96-Well $575.00
27259A Mouse Osteopontin N-Half Assay Kit - IBL 96-Well $575.00
 

 



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