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Products >  Cell_Biology >  Cancer_and_Inflammation >  ELISA_Kits_N-Z >  Napsin_A_Human

Human Napsin A ELISA Kit

Novel Aspartic Proteinase of the PepSIN Family (Napsin A, or NAPSA) belongs to the peptidase A1 family and plays a role in pneumocyte surfactant processing. It is also known as aspartyl protease 4 (ASP4), KAP, Kdap, napsin-1, NAP1, NAPA, and SNAPA. Two closely related proteins are known, Napsin A and Napsin B. Napsin A is a single-chain, 38 kDa protein. It is expressed at high levels in human lung and kidney, and at lower levels in spleen. Napsin A expression has been detected in type II pneumocytes and in lung adenocarcinomas.

This kit is a solid-phase sandwich ELISAs using two antibodies that are highly specific to human Napsin A protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This kit can be used to measure soluble human Napsin A protein in serum, urine, or cell culture supernatant.

At-A-Glance Documents Images & Data

Features

  • Capable of measuring human Napsin A
  • Sensitivity: 2.92 ng/mL

Applications

  • Measuring human Napsin A protein in serum, urine, or cell culture supernatant

Components

Precoated plate: Anti-Human Napsin A (61A2) Mouse IgG
MoAb Affinity-Purified
96 Well x 1
Labeled target: HRP conjugated Anti-Human Napsin A
(TMU-Ad02) Mouse IgG MoAb Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Human Napsin A0.5 mL x 2
EIA buffer: 30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 3.91–250 ng/mL
  • Assay sensitivity: 2.92 ng/mL
  • Assay duration: Overnight
  • Specificity: Human Napsin A
  • Species cross-reactivity: n/a
  • Test specimen type: Serum, urine, or cell culture supernatant
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Package Size Price # of Units Select
27758A Human Napsin A Assay Kit-IBL 96-Well $849.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


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