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Products >  Cell_Biology >  Cancer_and_Inflammation >  ELISA_Kits_N-Z >  N-ERC_Mesothelin

N-ERC/Mesothelin ELISA Kit

These kits are solid-phase sandwich ELISAs that include two antibodies that are highly specific to the 31-kDa fragment of human, rat, or mouse mesothelin (N-ERC); one is precoated on the ELISA plate and the other is HRP-conjugated. These assay kits can be used to measure soluble human, rat, or mouse mesothelin in serum, EDTA plasma, and cell culture supernatant samples.

The MSLN gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor (MPF) and mesothelin. Mesothelin is a glycosylphosphatidylinositol (GPI)-anchored cell-surface protein that may function as a cell adhesion protein. Mesothelin is cleaved by a furin-like protease to yield fragments approximately 31 kDa and 40 kDa. In humans, mesothelin is overexpressed in epithelial mesotheliomas, ovarian cancers, and in some types of squamous cell carcinomas. The rat expressed in renal carcinoma (ERC) gene, which was identified in a rat model of renal cancer, is a homolog of the human MSLN gene. It plays a role in cell adhesion and shape. Rat mesothelin was also shown to be dynamically expressed in the developing pancreas. In rat and mouse, mesothelin is also known as ERC/mesothelin, protein expressed in renal carcinoma, and pre-pro-megakaryocyte-potentiating factor.


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Features

  • Kits for measuring human, rat, or mouse mesothelin protein
  • Sensitivity: 0.01 ng/mL (Cat.# 27765); 0.15 ng/mL (Cat.# 27783); 0.017 ng/mL (Cat. #27190)

Applications

  • Measuring human, rat, or mouse mesothelin in serum, EDTA plasma, and cell culture supernatant samples

Components

Precoated plate: Anti-ERC Antibody Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-ERC Antibody
Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant N-ERC/Mesothelin0.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 0.08–5 ng/mL (Cat.# 27765); 0.5–32 ng/mL (Cat.# 27783); 0.07 - 4.4 ng/mL (Cat. # 27190)
  • Assay sensitivity: 0.01 ng/mL (Cat.# 27765); 0.15 ng/mL (Cat.# 27783); 0.017 ng/mL (Cat. # 27190)
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: ≤0.1% cross-reactivity with rat MCP-1, TNF-alpha, and osteopontin (Cat.# 27765); <0.1% cross-reactivity with mouse C-ERC/Mesothelin (Cat.# 27783); <0.1% cross-reactivity with human C-ERC/Mesothelin and mouse N-ERC/Mesothelin (Cat. # 27190)
  • Test specimen type: Serum, EDTA plasma, and cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
New Cat. # Product Contents Size Price Units Select
  27765A Rat N-ERC/Mesothelin Assay Kit - IBL 96-Well $606.00 *
  27783A Mouse N-ERC/Mesothelin Assay Kit - IBL 96-Well $849.00 *
27190A Human N-ERC/Mesothelin Assay Kit - IBL 96-Well $930.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 

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