These kits are solid-phase sandwich ELISAs that include two antibodies that are highly specific to the 31-kDa fragment of human, rat, or mouse mesothelin (N-ERC); one is precoated on the ELISA plate and the other is HRP-conjugated. These assay kits can be used to measure soluble human, rat, or mouse mesothelin in serum, EDTA plasma, and cell culture supernatant samples.
The MSLN gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor (MPF) and mesothelin. Mesothelin is a glycosylphosphatidylinositol (GPI)-anchored cell-surface protein that may function as a cell adhesion protein. Mesothelin is cleaved by a furin-like protease to yield fragments approximately 31 kDa and 40 kDa. In humans, mesothelin is overexpressed in epithelial mesotheliomas, ovarian cancers, and in some types of squamous cell carcinomas. The rat expressed in renal carcinoma (ERC) gene, which was identified in a rat model of renal cancer, is a homolog of the human MSLN gene. It plays a role in cell adhesion and shape. Rat mesothelin was also shown to be dynamically expressed in the developing pancreas. In rat and mouse, mesothelin is also known as ERC/mesothelin, protein expressed in renal carcinoma, and pre-pro-megakaryocyte-potentiating factor.