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Products >  Cell_Biology >  Cancer_and_Inflammation >  ELISA_Kits_A-M >  LRG

LRG1 ELISA Kits

Leucine-rich alpha2-glycoprotein 1 (LRG1; also known as LRG or HMFT1766) was first identified as a trace component in human serum. LRG1 contains a 24 amino-acid consensus sequence, the leucine-rich repeat (LRR), that is found in a large family of proteins. LRG is expressed in primary human neutrophils, and its expression is upregulated during neutrophilic granulocyte differentiation of human and murine cell lines. High levels of LRG1 have been reported in the cerebrospinal fluid of patients with idiopathic normal pressure hydrocephalus (iNPH), and LRG1 measurement can be useful to distinguish iNPH from Alzheimer's disease symptoms.

These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to human, mouse, or rat LRG1 protein; one is precoated on the ELISA plate and the other is HRP-conjugated. These kits can be used to measure soluble human or mouse LRG1 protein in serum, EDTA plasma, urine, or cerebrospinal fluid.

At-A-Glance Documents Images & Data

Features

  • Capable of measuring LRG1 protein
  • Sensitivity: 0.17 ng/mL (Cat.# 27769); 0.06 ng/mL (Cat.# 27785); 1.68 ng/mL (Cat.# 27770)

Applications

  • Measuring human, mouse, or rat LRG1 protein in serum, EDTA plasma, urine, or cerebrospinal fluid

Components

Precoated plate: Anti-LRG Rabbit IgG Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-LRG Rabbit IgG Fab
Affinity-Purified
0.4 mL x 1
Standard: Recombinant LRG0.5 mL x 2
EIA buffer:30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 1.56–100 ng/mL (Cat.# 27769); 0.25–16 ng/mL (Cat.# 27785); 6.25 - 400 ng/mL (Cat.# 27770)
  • Assay sensitivity: 0.17 ng/mL (Cat.# 27769); 0.06 ng/mL (Cat.# 27785); 1.68 ng/mL (Cat.# 27770)
  • Assay duration: Overnight
  • Specificity: Human (Cat.# 27769), mouse (Cat.# 27785), or rat (Cat.# 27770) LRG1
  • Species cross-reactivity: n/a
  • Test specimen type: Serum, EDTA plasma, urine, or cerebrospinal fluid
  • Sample volume per well: 100 µL



 
 
Products
Cat. # Product Package Size Price # of Units Select
27785A Mouse LRG Assay Kit - IBL 96-Well $750.00
27769A Human LRG Assay Kit - IBL 96-Well $575.00
27770A Rat LRG Assay Kit - IBL 1 x 96-Well Plate $874.00
 

 



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