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Products >  Cell_Biology >  Cancer_and_Inflammation >  ELISA_Kits_A-M >  Intelectin-1_Omentin-1

Human Intelectin-1/Omentin-1 ELISA Kit

Lectins are sugar-binding proteins that play a role in biological recognition of specific sugar-containing molecules. Human intelectin-1 (encoded by the ITLNI gene) was first identified by its binding to galactofuranosyl residues. Intelectin-1 functions as a receptor for both bacterial arabinogalactans and lactoferrin. It has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. Recently, it was found that intelectin-1 may be a useful molecular marker for epithelioid-type malignant pleural mesotheliomas (MPMs).

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to human intelectin-1 protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble human intelectin-1 protein in serum, heparin plasma, or cell culture supernatants.

At-A-Glance Documents

Features

  • Capable of measuring human intelectin-1 protein
  • Sensitivity: 0.23 ng/mL

Applications

  • Measuring human intelectin-1 protein in serum, heparin plasma, or cell culture supernatants

Components

Precoated plate: Anti-Human Intelectin-1 (2D11) Mouse
IgG MoAb Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Human
Intelectin-1 (2D2) Mouse IgG MoAb Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Human Intelectin-1/Omentin-10.5 mL x 2
EIA buffer:50 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution:  1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 0.31–20 ng/mL
  • Assay sensitivity: 0.23 ng/mL
  • Assay duration: Approximately 3–3.5 hours
  • Test specimen type: Serum, heparin plasma, or cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Package Size Price # of Units Select
27361A Human Intelectin-1/Omentin-1 Assay Kit - IBL 1 x 96-Well Plate $930.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


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