The c-Met ELISA is a solid-phase sandwich ELISA that uses two antibodies that are highly specific to human c-Met; one is a mouse monoclonal antibody that is precoated onto the c-Met ELISA plate and the other is an anti-human mouse antibody that is HRP-conjugated. The c-Met (HGFR) assay can be used to measure soluble human HGFR protein in serum, EDTA plasma or cell culture supernatants.
The c-Met proto-oncogene encodes a protein known as hepatocyte growth factor receptor HGFR. HGFR (also known as RCCP2, scatter factor (SF) receptor and AUTS9) is a heterodimeric protein composed of one alpha and one beta subunit. The highly glycosylated alpha subunit contains the extracellular domain of HGFR, while the beta subunit contains the protein's transmembrane domain. HGFR possesses tyrosine kinase activity and is expressed mainly in epithelial cells. The protein is involved in the activation of several oncogenic pathways as well as in tumor metastasis and angiogenesis.