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Products >  Cell_Biology >  Cancer_and_Inflammation >  ELISA_Kits_A-M >  Human_c-Met_ELISA

c-Met ELISA

The c-Met ELISA is a solid-phase sandwich ELISA that uses two antibodies that are highly specific to human c-Met; one is a mouse monoclonal antibody that is precoated onto the c-Met ELISA plate and the other is an anti-human mouse antibody that is HRP-conjugated. The c-Met (HGFR) assay can be used to measure soluble human HGFR protein in serum, EDTA plasma or cell culture supernatants.

The c-Met proto-oncogene encodes a protein known as hepatocyte growth factor receptor HGFR. HGFR (also known as RCCP2, scatter factor (SF) receptor and AUTS9) is a heterodimeric protein composed of one alpha and one beta subunit. The highly glycosylated alpha subunit contains the extracellular domain of HGFR, while the beta subunit contains the protein's transmembrane domain. HGFR possesses tyrosine kinase activity and is expressed mainly in epithelial cells. The protein is involved in the activation of several oncogenic pathways as well as in tumor metastasis and angiogenesis.

At-A-Glance Documents Images & Data

Features

Cat. #27407A

  • Capable of measuring human c-Met HGFR protein
  • Sensitivity: 0.26 ng/mL

Applications

  • Measurement of human c-Met HGFR protein in cell culture supernatants

Components

Precoated plate: Anti-Human c-Met Mouse IgG MoAb
Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Human c-Met
Mouse IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Human c-Met0.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 0.78–50 ng/mL
  • Assay sensitivity: 0.26 ng/mL
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Human c-Met (HGFR)
  • Test specimen type: Serum, EDTA plasma, or cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Package Size Price # of Units Select
27407A Human c-Met Assay Kit - MCM 96-Well $1,867.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


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