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Products >  Cell_Biology >  Cancer_and_Inflammation >  ELISA_Kits_A-M >  Human_Syndecan-4_ELISA

Human Syndecan-4 ELISA Kit

Syndecans are a family of plasma membrane proteoglycans, normally found on the cell surface of fibroblasts and epithelial cells. Syndecans interact with fibronectin on the cell surface, and with cytoskeletal and signaling proteins inside the cell, and play important roles in pathways related to cell-matrix adhesion. Syndecan-4 (also known as ryudocan, amphiglycan, and SYND4) has a molecular weight of 20 kDa. It interacts with fibronectin on the cell surface and recruits fibroblast growth factor (FGF) to its receptor. The cytoplasmic portion of syndecan-4 interacts with focal adhesion molecules, suggesting a role for syndecan-4 in anticoagulation, cell adhesion, and cell multiplication.

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to human syndecan-4 protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble human syndecan-4 protein in serum, EDTA plasma, or cell culture supernatants.

At-A-Glance Documents Images & Data

Features

  • Capable of measuring human syndecan-4 protein
  • Sensitivity: 3.94 pg/mL

Applications

  • Measuring human syndecan-4 protein in serum, EDTA plasma, or cell culture supernatants

Components

Precoated plate: Anti-Human Syndecan-4 Rabbit IgG
Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Human
Syndecan-4 Rabbit IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Human Syndecan-40.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 20–1280 pg/mL
  • Assay sensitivity: 3.94 pg/mL
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Human syndecan-4
  • Test specimen type: Serum, EDTA plasma, or cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Package Size Price # of Units Select
27188A Human Syndecan-4 Assay Kit - IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


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