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Products >  Cell_Biology >  Cancer_and_Inflammation >  ELISA_Kits_A-M >  Human_Osteopontin_ELISA

Osteopontin ELISA

The Osteopontin ELISA is a solid-phase sandwich ELISA that uses two highly specific antibodies for human, mouse or rat osteopontin (OPN) protein; one of the antibodies is precoated onto the osteopontin ELISA plate and the other is HRP-conjugated. The osteopontin ELISA kit can be used to measure soluble human, mouse or rat OPN protein in EDTA-plasma, cell culture supernatant or urine.

OPN is a calcium-binding glycosylated phosphoprotein secreted by many cell types, including osteoblasts, kidney tubule cells, macrophages, activated T cells and vascular smooth muscle cells. It is also known as Spp-1, Apl-1, BNSP, bone sialoprotein 1, ETA-1 and minopontin. OPN molecular weight varies within a range of 44-66 kDa depending on the protein's level of glycosylation and phosphorylation. OPN contains an Arg-Gly-Asp (RGD) amino acid motif that is also found in other extracellular proteins that bind members of the integrin family of cell-surface receptors. A variety of OPN isoforms exists within the cell and is produced by differential splicing, post-translational modification and proteolytic cleavage.

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Features

Human Osteopontin Assay Kit  (Cat.#: 27158A):

  • Specific for human OPN. Cross-reactivity with mouse OPN: 0.2%. Cross-reactivity with rat OPN: less than or equal to 0.1%
  • Sensitivity of 3.33 ng/mL

Mouse Osteopontin Assay Kit  (Cat.#: 27351A):

  • Specificity: 100% cross-reaction with mouse OPN. 14.8% cross-reaction with rat OPN. 4.3% cross-reaction with human OPN.
  • Sensitivity of 0.15 ng/mL

Rat Osteopontin Assay Kit  (Cat.#: 27360A):

  • Specificity: 100% cross-reaction with rat OPN. Cross-reaction with rat or human OPN less than or equal to 0.1%.
  • Sensitivity of 0.01 ng/mL

Applications

  • Measurement of human, mouse or rat OPN protein in EDTA-plasma, cell culture supernatant or urine

Components

Precoated plate: Anti-OPN Rabbit IgG Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-OPN IgG Fab
Affinity-Purified
0.4 mL x 1
Standard: Recombinant Human, Mouse or Rat OPN0.5 mL x 2
EIA buffer : 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 5–320 ng/mL (Cat.# 27158); 1–64 ng/mL (Cat.# 27351); 0.07–4.75 ng/mL (Cat.# 27360)
  • Assay sensitivity: 3.33 ng/mL (Cat.# 27158); 0.15 ng/mL (Cat.# 27351); 0.01 ng/mL (Cat.# 27360)
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Human, mouse or rat OPN
  • Species cross-reactivity: 0.1%–14.8% cross-reactivity among human, mouse and rat OPN
  • Test specimen type: EDTA-plasma, cell culture supernatant or urine
  • Sample volume per well: 100 µL

 
Products
Offers Cat. # Product Contents Size Price Units Select
27158A Human Osteopontin Assay Kit - IBL 96-Well $606.00 *
27351A Mouse Osteopontin Assay Kit - IBL 96-Well $606.00 *
27360A Rat Osteopontin Assay Kit - IBL 96-Well $606.00 *
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