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Products >  Cell_Biology >  Cancer_and_Inflammation >  ELISA_Kits_A-M >  HGF_Total_Human

Human Total HGF ELISA Kit

Hepatocyte growth factor (HGF) belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. HGF is also known as hepapoietin A, hepatopoeitin A, scatter factor (SF), fibroblast-derived tumor cytotoxic factor (F-TCF), HGFB, HPTA, and deafness, autosomal recessive 39 (DFNB39). It is secreted as a single inactive polypeptide that is cleaved by a serine protease (known as hepatocyte growth factor activator, or HGFA) into a 69 kDa alpha chain and 34 kDa beta chain. Active HGF is a heterodimer between the alpha and beta chains, linked by a disulfide bond. HGF acts as a multifunctional cytokine in cells of epithelial origin. It regulates cell growth, motility, and morphogenesis by binding to the c-Met receptor and activating a tyrosine kinase signaling cascade. HGF also plays a role in tissue regeneration, angiogenesis, and tumorigenesis.

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to human HGF protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble human HGF protein in serum, EDTA plasma, or cell culture supernatants.

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Features

  • Capable of measuring total human HGF
  • Sensitivity: 11.0 pg/mL

Applications

  • Measuring total human HGF protein in serum, EDTA plasma, or cell culture supernatants

Components

Precoated plate: Anti-Human Total HGF Rabbit IgG
Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Human
HGF Goat IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Human Total HGF0.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 78.13–5000 pg/mL
  • Assay sensitivity: 11.0 pg/mL
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Exhibits ≤0.1% cross-reactivity with human HAI-1, HAI-2, c-Met, and HGFA
  • Test specimen type: Serum, EDTA plasma, or cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
Offers Cat. # Product Contents Size Price Units Select
27402A Human Total HGF Assay Kit - MCM 96-Well $1,867.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 

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