Home Takara Clontech Logo Takara Clontech
login | register | order now | view cart | feedback
Products
Cell Biology


Products >  Cell_Biology >  Cancer_and_Inflammation >  ELISA_Kits_A-M >  Galectin-3_Human

Human Galectin-3 ELISA Kit

Galectin-3 belongs to a family of carbohydrate-binding proteins known as lectins. In humans, it is encoded by the LGALS3 gene. Galectin-3 has a N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. It binds beta-galactoside and IgE, and is required for terminal differentiation of columnar epithelial cells during early embryogenesis. Galectin-3 is involved in a broad range of functions, including innate immunity, chemoattraction, cell adhesion, cell growth and differentiation, T-cell regulation, and apoptosis. High levels of galectin-3 expression have been associated with heart failure, cancer, and inflammation. Galectin-3 is also known as lectin, galactoside-binding, soluble, 3; carbohydrate-binding protein 35, galactose-specific lectin 3, laminin-binding protein, lectin L-29, Mac-2 antigen, IgE-binding protein, L31, GAL3, MAC2, CBP35, GALBP, GALIG, and LGALS2.

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to human galectin-3 protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble human galectin-3 protein in serum, EDTA plasma, cell and tissue lysates, and cell culture supernatants.

  At-A-Glance   Documents   Images & Data

Features

  • Capable of measuring human galectin-3 protein
  • Sensitivity: 43.95 pg/mL

Applications

  • Measuring human galectin-3 protein in serum, EDTA plasma, cell and tissue lysates, and cell culture supernatants

Components

Precoated plate: Anti-Human Galectin-3 (50A3) Mouse IgG MoAb
Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Human Galectin-3
(87B5) Mouse IgG MoAb Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Human Galectin-30.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 117.19–7500 pg/mL
  • Assay sensitivity: 43.95 pg/mL
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Shows ≤0.1% cross-reactivity with human galectin-1 and mouse galectin-3; 0.21% with human galectin-4
  • Test specimen type: Serum, EDTA plasma, cell and tissue lysates, and cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Contents Size Price Units Select
27755A Human Galectin-3 Assay Kit - IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 

Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions