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Products >  Cell_Biology >  Cancer_and_Inflammation >  ELISA_Kits_A-M >  COX-2

COX-2 Assay

The COX-2 Assay is a solid-phase sandwich ELISA that uses two highly specific antibodies to human or rat COX-2 protein; one antibody is precoated onto the COX-2 assay plate and the other antibody is HRP-conjugated. The COX-2 ELISA can be used to measure soluble human or rat COX-2 protein in cell culture lysates.

Cyclooxygenase (COX) is a membrane-bound enzyme that exists as two different isoforms: constitutive (COX-1) and inducible (COX-2). Also known as prostaglandin endoperoxide H synthase (PHS-2 or PGHS-2) and Tis10, COX-2 converts arachidonic acid to prostaglandin endoperoxide H2 in a two-step reaction. Levels of COX-2 are elevated during inflammation and in many forms of cancer. Nonsteroidal anti-inflammatory drugs (NSAIDs) inhibit prostaglandin production by both COX-1 and COX-2.


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Features

  • Capable of measuring human or rat COX-2
  • Sensitivity of 0.25 ng/mL (Cat.# 27186); 0.16 ng/mL (Cat.# 27187)

Applications

  • Measurement of human or rat COX-2 protein in cultured cell lysates.

Components

Precoated plate: Anti-COX-2 IgG Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP-conjugated Anti-COX-2 IgG Fab
Affinity-Purified
0.4 mL x 1
Standard: Recombinant COX-20.5 mL x 2
EIA buffer:30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 1.09–70 ng/mL (Cat.# 27186); 0.36–23 ng/mL (Cat.# 27187)
  • Assay sensitivity: 0.25 ng/mL (Cat.# 27186); 0.16 ng/mL (Cat.# 27187)
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Human (Cat.# 27186) or rat (Cat.# 27187) COX-2
  • Test specimen type: Cultured cell lysates
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Contents Size Price Units Select
27186A Human COX-2 Assay Kit - IBL 96-Well $606.00 *
27187A Rat COX-2 Assay Kit - IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 

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