The COX-2 Assay is a solid-phase sandwich ELISA that uses two highly specific antibodies to human or rat COX-2 protein; one antibody is precoated onto the COX-2 assay plate and the other antibody is HRP-conjugated. The COX-2 ELISA can be used to measure soluble human or rat COX-2 protein in cell culture lysates.
Cyclooxygenase (COX) is a membrane-bound enzyme that exists as two different isoforms: constitutive (COX-1) and inducible (COX-2). Also known as prostaglandin endoperoxide H synthase (PHS-2 or PGHS-2) and Tis10, COX-2 converts arachidonic acid to prostaglandin endoperoxide H2 in a two-step reaction. Levels of COX-2 are elevated during inflammation and in many forms of cancer. Nonsteroidal anti-inflammatory drugs (NSAIDs) inhibit prostaglandin production by both COX-1 and COX-2.