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Products >  Cell_Biology >  Cancer_and_Inflammation >  Antibodies_T-Z >  Tie-1_Human

Anti-Human Tie-1 Mouse IgG MoAb

Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE-1) is a tyrosine kinase cell-surface receptor that is expressed primarily in endothelial cells. It exhibits high homology to TEK/TIE-2 and inhibits the binding of TEK/TIE-2 to the growth factor angiopoietin-1. The extracellular domain of TIE-1 can be cleaved by multiple factors, including vascular endothelial growth factor (VEGF); this results in loss of its ability to inhibit TEK/TIE-2. In addition to its role in angiogenesis, TIE-1 is reported to have proinflammatory effects in endothelial cells. TIE-1 is also known as tyrosine-protein kinase receptor Tie-1, TIE, and JTK14.

These products are affinity-purified IgG antibodies that recognize human TIE-1 protein. The antibodies were raised in rabbit or mouse using recombinant protein or a synthetic peptide and can be used for Western blot (WB) detection, immunohistochemical (IHC) detection, immunoprecipitation (IP), or flow cytometry (FCM) of human TIE-1 protein.

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Features

  • Cat.#: 10353A, 10353B, 10353F
  • Target Name: Human TIE-1 protein
  • Antigen: Human TIE-1 (extracellular domain) recombinant protein
  • Application: WB, IP, FCM
  • Target Entrez Gene ID: 7075

  • Cat.#: 18541A, 18541B
  • Target Name: Human TIE-1 protein
  • Antigen: Human TIE-1 synthetic peptide
  • Application: WB, IHC
  • Target Entrez Gene ID: 7075

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 1–5 µg/mL (Cat.# 10353); 2–10 µg/mL (Cat.# 18541)
  • Immunohistochemical techniques: 5–10 µg/mL
  • Immunoprecipitation: 3–5 µg per reaction
  • Flow cytometry: 0.1–0.5 µg per 1 x 105 cells

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.*

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O.*

Alternative names

Tyrosine kinase with immunoglobulin-like and EGF-like domains 1, tyrosine-protein kinase receptor Tie-1, TIE, JTK14




 
 
Products
Cat. # Product Contents Size Price Units Select
10353A Anti-Human Tie-1 (9C1) Mouse IgG MoAb 10 ug $113.00 *
10353B Anti-Human Tie-1 (9C1) Mouse IgG MoAb 100 ug $499.00
10353F Anti-Human Tie-1 (9C1) Mouse IgG MoAb (Frozen) 100 ug $499.00
18541A Anti-Human Tie-1 (N1125) Rabbit IgG Affinity Purify 10 ug $113.00 *
18541B Anti-Human Tie-1 (N1125) Rabbit IgG Affinity Purify 100 ug $499.00
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 
 

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