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Products >  Cell_Biology >  Cancer_and_Inflammation >  Antibodies_O-S >  p53_Human_Monoclonal

Anti-Human p53 (Bp53-12) Mouse IgG MoAb

Tumor protein p53, encoded in humans by the TP53 gene, was originally identified based on its interaction with the large T antigen of simian virus 40 (SV40). p53 is expressed at low levels in most cell types but is upregulated in many transformed (cancer) cell lines. In response to cellular stress, p53 regulates over 100 target genes that control cell cycle arrest, apoptosis, senescence, DNA repair, and metabolic changes. p53 protein has multiple domains that include DNA-binding, transactivation, and oligomerization activities. Mutations in the TP53 gene cause loss of tumor suppression activity and are found in more than 50% of human tumors. Multiple isoforms of p53 are known, with distinct DNA-binding and transcriptional activation properties. p53 is also known as cellular tumor antigen p53, p53 tumor suppressor, transformation-related protein 53, BCC7, LFS1, TRP53, and antigen NY-CO-13.

This product is an affinity-purified IgG antibody that recognizes human p53 protein. The antibody was raised in mouse using recombinant p53 protein and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of human p53 protein.

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Features

  • Cat.#: 10041A, 10041B
  • Target Name: Human p53 protein
  • Antigen: Human p53 recombinant protein
  • Application: WB, IHC
  • Specificity: Reacts with both wild-type and mutant human p53 protein; does not cross-react with mouse or rat p53
  • Target Entrez Gene ID: 7157

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 2–5 µg/mL
  • Immunohistochemical techniques: 2–5 µg/mL

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O.

Alternative names

TP53, cellular tumor antigen p53, p53 tumor suppressor, transformation-related protein 53, BCC7, LFS1, TRP53, antigen NY-CO-13




 
 
Products
Offers Cat. # Product Contents Size Price Units Select
10041A Anti-Human p53 (Bp53-12) Mouse IgG MoAb 200 ug $499.00
  10041B Anti-Human p53 (Bp53-12) Mouse IgG MoAb 500 ug $1,000.00
 

 

 
 

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