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Products >  Cell_Biology >  Cancer_and_Inflammation >  Antibodies_O-S >  Smad3

Human SMAD3 Antibody (Phosphorylated)

The SMAD family of proteins in humans are homologs of Drosophila 'mothers against decapentaplegic' and C. elegans Sma proteins. SMAD2 and SMAD3 are intracellular signaling proteins and transcriptional regulators that are activated by transforming growth factor beta (TGF-beta). Phosphorylation of SMAD2/3 is useful for understanding of various biological functions of TGF-beta. TGF-beta type I receptor and cyclin-dependent kinases phosphorylate both SMAD2 and SMAD3 in C-terminal and linker regions. TGF-beta signaling is mediated through these phosphorylation events

These products are affinity-purified IgG antibodies that recognize different phosphorylated forms of human SMAD2/SMAD3 protein: C-terminally phosphorylated (pSMAD3C) and linker-phosphorylated (pSMAD3L, pSMAD2L). The antibodies were raised in rabbit or mouse using synthetic peptides and can be used for Western blot (WB) detection, immunohistochemical (IHC) detection, or immunoprecipitation (IP).

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Features

  • Cat.#: 28029A, 28029B
  • Target Name: Human SMAD3 protein
  • Antigen: Human pSMAD3L (Ser208/Ser213 phosphorylated) synthetic peptide
  • Specificity: Cross-reacts with rat and mouse SMAD3 (Ser 208/213 phosphorylated); does not cross-react with SMAD3 (Ser 204 phosphorylated), SMAD3 (Ser 245/250/255 phosphorylated), or nonphosphorylated SMAD3
  • Application: WB, IHC, IP
  • Target Entrez Gene ID: 4088

  • Cat.#: 28031A, 28031B
  • Target Name: Human SMAD3 protein
  • Antigen: Human pSMAD3C (Ser423/Ser425 phosphorylated) synthetic peptide
  • Specificity: Cross-reacts with rat and mouse SMAD3 (Ser 423/425 phosphorylated); cross-reacts with C-terminal phosphorylated SMAD2
  • Application: WB, IHC, IP
  • Target Entrez Gene ID: 4088

  • Cat.#: 10385A, 10385B
  • Target Name: SMAD2/3 protein
  • Antigen: Smad2L/3L (Thr220/Thr179 phosphorylated) synthetic peptide
  • Specificity: Reacts with human phosphorylated Smad3L and Smad2L (Thr220/Thr179)
  • Application: WB, IHC, IP
  • Target Entrez Gene ID: 4087, 4088

  • Cat.#: 10387A, 10387B
  • Target Name: SMAD3 protein
  • Antigen: Smad3L (Ser213 phosphorylated) synthetic peptide
  • Specificity: Reacts with human phosphorylated Smad3L (Ser21)
  • Application: WB, IHC, IP
  • Target Entrez Gene ID: 4088

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 0.2 µg/mL (Cat.# 28029); 0.4 µg/mL (Cat.# 28031); 2.0 µg/mL (Cat.# 10385 and 10387)
  • Immunohistochemical techniques: 2.0 µg/mL (Cat.# 28029, 10385, and 10387); 0.4 µg/mL (Cat.# 28031)
  • Immunoprecipitation: 0.5 µg per reaction (Cat.# 28029 and 28031); 2.0 µg/mL (Cat.# 10385 and 10387)

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3.To use, reconstitute in 1.0 mL H2O (final concentration 100 µg/mL).

Alternative names

MAD, mothers against decapentaplegic homolog 3 (Drosophila); mothers against DPP homolog 3, MADH3, hMAD-3, hSMAD3, MAD homolog 3, Mad3, LDS3, LDS1C, mad homolog JV15-2, JV15-2, HSPC193, HsT17436




 
 
Products
Offers Cat. # Product Contents Size Price Units Select
10385A Anti-Smad2L/3L (T220/T179 Phosphorylated) (1A1) Mouse IgG MoAb 5 ug $114.00 *
  10385B Anti-Smad2L/3L (T220/T179 Phosphorylated) (1A1) Mouse IgG MoAb 50 ug $525.00
  10387A Anti-Smad3L (S213 Phosphorylated) (5A11) Mouse IgG MoAb 5 ug $114.00 *
  10387B Anti-Smad3L (S213 Phosphorylated) (5A11) Mouse IgG MoAb 50 ug $525.00
28029A Anti-Smad3L (Ser 208/213 Phosphorylated) Rabbit IgG Affinity Purify 5 ug $113.00 *
28029B Anti-Smad3L (Ser 208/213 Phosphorylated) Rabbit IgG Affinity Purify 50 ug $499.00
28031A Anti-Smad3C (Ser 423/425 Phosphorylated) Rabbit IgG Affinity Purify 5 ug $113.00 *
28031B Anti-Smad3C (Ser 423/425 Phosphorylated) Rabbit IgG Affinity Purify 50 ug $499.00
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