The Poly (ADP-Ribose) Antibody is an affinity-purified IgG antibody that recognizes poly (ADP-ribose). The Poly (ADP-Ribose) Antibody is raised in mouse using purified poly (ADP-ribose) and can be used for Western blot (WB) and immunohistochemical (IHC) detection as well as immunocytochemical (ICC) detection of poly(ADP-ribosyl)ated proteins.
The addition of multiple ADP-ribose molecules to a protein, or poly (ADP-ribosyl)ation, is an important post-translational modification. This process is used in cell signaling and to regulate a variety of functions, including DNA damage repair, chromatin modification, transcription, and apoptosis1. In the nucleus, poly (ADP-ribosyl)ation is involved in mitotic apparatus function and telomere maintenance.
In most eukaryotes, poly(ADP-ribose) polymerases (PARPs) catalyze poly (ADP-ribosyl)ation through donor nicotinamide adenine dinucleotide (NAD+) molecules. Conversely, prokaryotes and yeast lack PARPs.