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Products >  Cell_Biology >  Cancer_and_Inflammation >  Antibodies_O-S >  Poly_ADP_Ribose

Poly (ADP-Ribose) Antibody

The Poly (ADP-Ribose) Antibody is an affinity-purified IgG antibody that recognizes poly (ADP-ribose). The Poly (ADP-Ribose) Antibody is raised in mouse using purified poly (ADP-ribose) and can be used for Western blot (WB) and immunohistochemical (IHC) detection as well as immunocytochemical (ICC) detection of poly(ADP-ribosyl)ated proteins.

The addition of multiple ADP-ribose molecules to a protein, or poly (ADP-ribosyl)ation, is an important post-translational modification. This process is used in cell signaling and to regulate a variety of functions, including DNA damage repair, chromatin modification, transcription, and apoptosis1. In the nucleus, poly (ADP-ribosyl)ation is involved in mitotic apparatus function and telomere maintenance.

In most eukaryotes, poly(ADP-ribose) polymerases (PARPs) catalyze poly (ADP-ribosyl)ation through donor nicotinamide adenine dinucleotide (NAD+) molecules. Conversely, prokaryotes and yeast lack PARPs.

At-A-Glance Documents

Features

Cat.#: 10407A, 10407B
  • Name: Poly (ADP-ribose)
  • Antigen: Purified poly (ADP-ribose)
  • Application: WB, IHC, ICC

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • WB: 2–5 µg/mL
  • IHC: 10 µg/mL
  • ICC: 10 µg/mL

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

References:

1. Kim, M.Y. et al. (2005) Genes & Dev. 19:1951-1967.


Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute each 10 ug vial in 100 ul H2O and each 100 ug vial in 1.0 mL H2O (final concentration, 100 µg/mL).




 
 
Products
Cat. # Product Package Size Price # of Units Select
10407A Anti-Poly (ADP-ribose) (10H) Mouse IgG MoAb 10 ug $128.00 *
10407B Anti-Poly (ADP-ribose) (10H) Mouse IgG MoAb 100 ug $525.00
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


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