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Products >  Cell_Biology >  Cancer_and_Inflammation >  Antibodies_O-S >  Osteopontin_O-17

Anti-Osteopontin (O-17) Rabbit IgG Antibody

Osteopontin (OPN) is a calcium-binding glycosylated phosphoprotein secreted by many cell types, including osteoblasts, kidney tubule cells, macrophages, activated T cells and vascular smooth muscle cells. It is also known as Spp-1, Apl-1, BNSP, bone sialoprotein 1, ETA-1, and minopontin. The molecular weight varies within the range of 44-66 kDa, depending on the level of glycosylation and phosphorylation. OPN contains an Arg-Gly-Asp (RGD) amino acid motif that is also found in other extracellular proteins that bind members of the integrin family of cell-surface receptors. A variety of OPN isoforms exist within the cell, produced by differential splicing, post-translational modification and proteolytic cleavage.

These products are affinity-purified IgG antibodies that recognize the N-terminus of OPN (O-17). The antibodies were raised in rabbit using a synthetic peptide and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of OPN.

At-A-Glance Documents Images & Data

Features

  • Cat.#: 18621A, 18621B
  • Target Name: Mouse OPN (O-17)
  • Antigen: Mouse OPN N-terminal synthetic peptide
  • Application: IHC, WB
  • Specificity: Cross-reacts with rat OPN
  • Target Entrez Gene ID: 6696

  • Cat.#: 18625A, 18625B
  • Target Name: Human OPN (O-17)
  • Antigen: Human OPN N-terminal synthetic peptide
  • Application: IHC, WB
  • Specificity: Does not cross-react with mouse/rat OPN
  • Target Entrez Gene ID: 6696

  • Cat.#: 18628A, 18628B
  • Target Name: Rat OPN (O-17)
  • Antigen: Rat OPN N-terminal synthetic peptide
  • Application: IHC, WB
  • Specificity: Does not cross-react with mouse or human OPN
  • Target Entrez Gene ID: 6696

Applications:

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Immunohistochemical techniques: 1-2 µg/mL
  • Western blot: 1–5 µg/mL

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O (final concentration, 100 µg/mL)

Alternative names

OPN, Spp-1, Apl-1, BNSP, bone sialoprotein 1, BSPI, ETA-1, minopontin




 
 
Products
Cat. # Product Package Size Price # of Units Select
18621A Anti-Mouse Osteopontin (O-17) Rabbit IgG Affinity Purify 10 ug $100.00
18621B Anti-Mouse Osteopontin (O-17) Rabbit IgG Affinity Purify 100 ug $499.00
18625A Anti-Human Osteopontin (O-17) Rabbit IgG Affinity Purify 10 ug $100.00
18625B Anti-Human Osteopontin (O-17) Rabbit IgG Affinity Purify 100 ug $499.00
18628A Anti-Rat Osteopontin (O-17) Rabbit IgG Affinity Purify 10 ug $100.00
18628B Anti-Rat Osteopontin (O-17) Rabbit IgG Affinity Purify 100 ug $499.00
 

 



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