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Products >  Cell_Biology >  Cancer_and_Inflammation >  Antibodies_O-S >  Osteopontin_N-Half_Monoclonal

Anti-Osteopontin N-Half (34E3) Mouse IgG MoAb

Osteopontin (OPN) is a calcium-binding glycosylated phosphoprotein secreted by many cell types. It is expressed in osteoblasts, kidney tubule cells, macrophages, activated T cells, and vascular smooth muscle cells. Osteopontin is post-translationally modified; the molecular weight varies within the range of 44-66 kDa, depending on the level of glycosylation and phosphorylation. OPN contains an Arg-Gly-Asp (RGD) amino acid motif that is also found in other extracellular proteins that bind members of the integrin family of cell-surface receptors. This binding activity has been localized to the N-terminal fragment of osteopontin, generated by thrombin cleavage. Osteopontin is also known as Spp-1, Apl-1, BNSP, bone sialoprotein 1, ETA-1, and minopontin.

This product is an affinity-purified IgG antibody that recognizes the N-terminal fragment of human OPN. The antibody was raised in mouse using a synthetic peptide and can be used for Western blot (WB) detection of human OPN.

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Features

  • Cat.#: 11108A, 11108B
  • Target Name: Human OPN (N-terminal fragment)
  • Antigen: Human OPN N-terminal synthetic peptide
  • Application: WB
  • Specificity: Cross-reacts with rat and mouse N-terminal OPN fragment; does not cross-react with full-length OPN
  • Target Entrez Gene ID: 6696

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 1–5 µg/mL

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O.

Alternative names

OPN, Spp-1, Apl-1, BNSP, bone sialoprotein 1, BSPI, ETA-1, minopontin




 
 
Products
Offers Cat. # Product Contents Size Price Units Select
11108A Anti-Osteopontin N-Half (34E3) Mouse IgG MoAb 10 ug $113.00 *
  11108B Anti-Osteopontin N-Half (34E3) Mouse IgG MoAb 100 ug $499.00
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 
 

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