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Products >  Cell_Biology >  Cancer_and_Inflammation >  Antibodies_O-S >  Osteopontin_Human_Monoclonal

Anti-Human Osteopontin Mouse IgG MoAb

Osteopontin (OPN) is a calcium-binding glycosylated phosphoprotein secreted by many cell types, including osteoblasts, kidney tubule cells, macrophages, activated T cells, and vascular smooth muscle cells. It is also known as Spp-1, Apl-1, BNSP, bone sialoprotein 1, ETA-1, and minopontin. The molecular weight varies within the range of 44-66 kDa, depending on the level of glycosylation and phosphorylation. OPN contains an Arg-Gly-Asp (RGD) amino acid motif that is also found in other extracellular proteins that bind members of the integrin family of cell-surface receptors. A variety of OPN isoforms exist within the cell, produced by differential splicing, post-translational modification and proteolytic cleavage.

These products are affinity-purified IgG antibodies that recognize human OPN. The antibodies were raised in mouse using a synthetic peptide and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of human OPN.

At-A-Glance Documents Images & Data

Features

  • Cat.#: 10011A
  • Target Name: Human OPN
  • Antigen: Human OPN synthetic peptide
  • Clone: 10A16
  • Application: IHC, WB
  • Specificity: Does not cross-react with mouse/rat OPN
  • Target Entrez Gene ID: 6696

  • Cat.#: 10191A, 10191B
  • Target Name: Human OPN
  • Antigen: Human OPN C-terminal synthetic peptide
  • Clone: 1B20
  • Application: IHC, WB
  • Specificity: Cross-reacts with rabbit OPN
  • Target Entrez Gene ID: 6696

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Immunohistochemical techniques: 1–2 µg/mL
  • Western blot: 2 µg/mL

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O (final concentration, 100 µg/mL)

Alternative names

OPN, Spp-1, Apl-1, BNSP, bone sialoprotein 1, BSPI, ETA-1, minopontin




 
 
Products
Cat. # Product Package Size Price # of Units Select
10011A Anti-Human Osteopontin (10A16) Mouse IgG MoAb 200 ug $499.00
10191A Anti-Human Osteopontin (1B20) Mouse IgG MoAb 10 ug $113.00 *
10191B Anti-Human Osteopontin (1B20) Mouse IgG MoAb 100 ug $499.00
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


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