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Products >  Cell_Biology >  Cancer_and_Inflammation >  Antibodies_H-N >  HGF_Alpha

Anti-HGF Rabbit IgG Antibody

Hepatocyte growth factor (HGF) belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. HGF is secreted by mesenchymal cells as a single inactive polypeptide that is cleaved by serine proteases into a 69 kDa alpha chain and 34 kDa beta chain. The active form of HGF is a heterodimer, produced by disulfide bond formation between the alpha and beta chains. HGF acts as a multifunctional cytokine in epithelial cells. It regulates cell growth, motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the c-Met receptor. HGF plays a role in tissue regeneration, angiogenesis, and tumorigenesis. HGF is also known as hepapoietin A, hepatopoeitin A, scatter factor (SF), fibroblast-derived tumor cytotoxic factor (F-TCF), HGFB, HPTA, and deafness, autosomal recessive 39 (DFNB39).

These products are affinity-purified IgG antibodies that recognize human or rat HGF protein. The antibodies were raised in rabbit using synthetic peptides, and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of human or rat HGF protein.

At-A-Glance Documents Images & Data

Features

  • Cat.#: 18131A, 18131B
  • Target Name: Human HGF (alpha chain)
  • Antigen: Human HGF alpha chain N-terminal synthetic peptide
  • Specificity: Reacts with human and rat HGF alpha chain; does not cross-react with human HGF beta chain
  • Application: WB, IHC
  • Target Entrez Gene ID: 3082

  • Cat.#: 18133A
  • Target Name: Human HGF (alpha chain)
  • Antigen: Human HGF alpha chain synthetic peptide
  • Specificity: Reacts with human and rat HGF alpha chain; does not cross-react with human HGF beta chain
  • Application: WB, IHC
  • Target Entrez Gene ID: 3082

  • Cat.#: 18135A, 18135B
  • Target Name: Human HGF (alpha chain)
  • Antigen: Human HGF alpha chain C-terminal synthetic peptide
  • Specificity: Reacts with human and rat HGF alpha chain; does not cross-react with human HGF beta chain
  • Application: WB, IHC
  • Target Entrez Gene ID: 3082

  • Cat.#: 18281A, 18281B
  • Target Name: Rat HGF (alpha chain)
  • Antigen: Rat HGF alpha chain N-terminal synthetic peptide
  • Specificity: Reacts with rat HGF alpha chain; does not cross-react with rat HGF beta chain, or human HGF alpha/beta chains
  • Application: WB, IHC
  • Target Entrez Gene ID: 24446

  • Cat.#: 18132A, 18132B
  • Target Name: Human HGF (beta chain)
  • Antigen: Human HGF beta chain C-terminal synthetic peptide
  • Specificity: Reacts with human HGF beta chain; does not cross-react with rat HGF
  • Application: WB, IHC
  • Target Entrez Gene ID: 3082

  • Cat.#: 18134A, 18134B
  • Target Name: Human HGF (beta chain)
  • Antigen: Human HGF beta chain N-terminal synthetic peptide
  • Specificity: Reacts with human and rat HGF beta chain; does not cross-react with human HGF alpha chain
  • Application: WB, IHC
  • Target Entrez Gene ID: 3082

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 2–5 µg/mL
  • Immunohistochemical techniques: 2–5 µg/mL

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O (final concentration 100 µg/mL).

Alternative names

hepapoietin A, hepatopoeitin A, scatter factor, SF, fibroblast-derived tumor cytotoxic factor, F-TCF, HGFB, HPTA; deafness, autosomal recessive 39; DFNB39




 
 
Products
Cat. # Product Package Size Price # of Units Select
18281A Anti-Rat HGF alpha(H56) Rabbit IgG Affinity Purify 10 ug $113.00 *
18281B Anti-Rat HGF alpha(H56) Rabbit IgG Affinity Purify 100 ug $499.00
18131A Anti-Human HGF alpha(H55) Rabbit IgG Affinity Purify 10 ug $113.00 *
18131B Anti-Human HGF alpha(H55) Rabbit IgG Affinity Purify 100 ug $499.00
18132A Anti-Human HGF beta (H714) Rabbit IgG Affinity Purify 10 ug $113.00 *
18132B Anti-Human HGF beta (H714) Rabbit IgG Affinity Purify 100 ug $499.00
18133A Anti-Human HGF alpha(H163) Rabbit IgG Affinity Purify 100 ug $499.00
18134A Anti-Human HGF beta (H495) Rabbit IgG Affinity Purify 10 ug $113.00 *
18134B Anti-Human HGF beta (H495) Rabbit IgG Affinity Purify 100 ug $499.00
18135A Anti-Human HGF alpha(H487) Rabbit IgG Affinity Purify 10 ug $113.00 *
18135B Anti-Human HGF alpha(H487) Rabbit IgG Affinity Purify 100 ug $499.00
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


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