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Products >  Cell_Biology >  Cancer_and_Inflammation >  Antibodies_A-G >  c-Kit_Human

Anti-Human c-Kit Antibody

c-Kit is a tyrosine kinase that exists both as a transmembrane protein and a soluble protein. It is the receptor for stem cell factor (SCF), and is also known as v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, stem cell factor receptor (SCFR), piebald trait protein (PBT), and CD117. Functional SCF and c-Kit are critical to the development and survival of hematopoietic stem cells. Interaction of SCF with c-Kit results in increased autophosphorylation activity. Multiple transcripts encoding different isoforms of c-Kit have been reported. High levels of c-Kit expression are found in some types of cancer cells, including gastrointestinal stromal tumors (GIST) and melanoma.

These products are affinity-purified IgG antibodies that recognize human c-Kit protein. The antibodies were raised in mouse or rabbit using a recombinant protein (Cat.# 10349A, 10349B, 10349F) or synthetic peptides (18101A, 18101B, 18105B) and can be used for Western blot (WB) detection (Cat.# 10349A, 10349B, 10349F, 18101A, 18101B), immunohistochemical (IHC) detection (Cat.# 18101A, 18101B, 18105B), immunoprecipitation (IP), or flow cytometry (FC) (10349A, 10349B, 10349F) of human c-Kit protein.

At-A-Glance Documents Images & Data

Features

  • Cat.#: 10349A, 10349B, 10349F
  • Target Name: Human c-Kit protein
  • Antigen: Human c-Kit (extracellular domain) recombinant protein
  • Clone: 12A8
  • Application: WB, IP, FC, Neutralization Assay
  • Specificity: Human c-Kit
  • Target Entrez Gene ID: 3815

  • Cat.#: 18101A, 18101B
  • Target Name: Human c-Kit protein
  • Antigen: Human c-Kit C-terminal synthetic peptide
  • Application: WB, IHC
  • Specificity: Human c-Kit
  • Target Entrez Gene ID: 3815

  • Cat.#: 18105B
  • Label: Antibody is FITC-conjugated
  • Target Name: Human c-Kit protein
  • Antigen: Human c-Kit C-terminal synthetic peptide
  • Application: IHC
  • Specificity: Human c-Kit
  • Target Entrez Gene ID: 3815

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 1–10 µg/mL
  • Immunohistochemical techniques: 1–10 µg/mL
  • Immunoprecipitation: 3–5 µg/mL
  • Flow cytometry: 0.1–0.5 µg (per 1 x 105 cells)

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3; frozen (Cat.# 10349F only). To use, reconstitute in 1.0 mL H2O.

Alternative names

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, stem cell factor receptor (SCFR), piebald trait protein (PBT), CD117




 
 
Products
Cat. # Product Package Size Price # of Units Select
10349A Anti-Human c-Kit (12A8) Mouse IgG MoAb 10 ug $113.00 *
10349B Anti-Human c-Kit (12A8) Mouse IgG MoAb 100 ug $499.00
10349F Anti-Human c-Kit (12A8) Mouse IgG MoAb (Frozen) 100 ug $499.00
18101A Anti-Human c-Kit (K963) Rabbit IgG Affinity Purify 10 ug $113.00 *
18101B Anti-Human c-Kit (K963) Rabbit IgG Affinity Purify 100 ug $499.00
18105B Anti-Human c-Kit (K963) Rabbit IgG Affinity Purify FITC 100 ug $499.00
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 



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