Takara
login | register | order now | view cart | feedback
Products
Cell Biology


Products >  Cell_Biology >  Brain_and_CNS >  Antibodies_O-S >  RGMa

Anti-RGMa Antibody

The repulsive guidance molecule (RGM) protein family performs several functions in the developing and adult nervous system. The three members--RGMa, RGMb, and RGMc--share a high level of protein sequence identity. RGMa is a glycosylphosphatidylinositol (GIP)-anchored glycoprotein that acts as an axon guidance protein during development of the central nervous system. RGMa regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and regulates the formation of mature synapses. RGMa binds to neogenin as its receptor; overexpression of neogenin and downregulation of RGMa in the developing embryonic neural tube induces apoptosis. RGMa also binds some members of the bone morphogenetic protein (BMP) family. RGMa is also known as RGM domain family, member A; and RGM.

These products are affinity-purified IgG antibodies that recognize human or rat RGMa protein. The antibodies were raised in rabbit or mouse using synthetic peptides and can be used for Western blot (WB) detection, immunoprecipitation (IP), or flow cytometry (FCM) of RGMa protein.

At-A-Glance Documents Images & Data

Features

  • Cat.#: 10377A, 10377B
  • Target Name: Human RGMa protein
  • Antigen: Human RGMa synthetic peptide
  • Application: WB, IP, FCM
  • Target Entrez Gene ID: 56963

  • Cat.#: 28045A, 28045B
  • Target Name: Rat RGMa protein
  • Antigen: Rat RGMa synthetic peptide
  • Application: WB, IP, FCM
  • Target Entrez Gene ID: 308739

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 0.5–2.0 µg/mL
  • Immunoprecipitation: 1–3 µg per reaction
  • Flow cytometry: 0.05–0.5 µg per 1 x 105 cells (Cat.# 10377); 0.05–0.5 µg per 1 x 106 cells (Cat.# 28045)

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3.To use, reconstitute in 1.0 mL H2O (final concentration 100 µg/mL).

Alternative names

RGM domain family, member A; RGM




 
 
Products
Cat. # Product Package Size Price # of Units Select
10377A Anti-Human RGMa (410-10) Mouse IgG MoAb 10 ug $113.00 *
10377B Anti-Human RGMa (410-10) Mouse IgG MoAb 100 ug $499.00
28045A Anti-Rat RGMa Rabbit IgG Affinity Purify 10 ug $113.00 *
28045B Anti-Rat RGMa Rabbit IgG Affinity Purify 100 ug $499.00
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions