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Products >  Cell_Biology >  Brain_and_CNS >  Antibodies_14-3-3 >  14-3-3_Zeta_Human

Anti-Human 14-3-3-Zeta Protein Rabbit IgG Antibody

The 14-3-3 family of conserved regulatory proteins is expressed in all eukaryotic cells and are encoded by seven genes in mammals. 14-3-3 proteins bind many types of signaling proteins, including kinases, phosphatases, and transmembrane receptors. More than 100 signaling proteins have been reported as 14-3-3 ligands. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. Elevated amounts of 14-3-3 proteins are found in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease. 14-3-3-zeta/delta, also known as tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide, YWHAZ, YWHAD, and KCIP-1, interacts with insulin receptor substrate 1 (IRS1), suggesting a role in regulating insulin sensitivity. Several transcript variants have been identified for the YWHAZ gene; they differ in the 5' UTR but encode the same protein.

This product is an affinity-purified IgG antibody that recognizes human 14-3-3-zeta protein. The antibody was raised in rabbit using a synthetic peptide, and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of human 14-3-3-zeta protein.

At-A-Glance Documents Images & Data

Features

  • Cat.#: 18644A, 18644B
  • Target Name: Human 14-3-3-zeta protein
  • Antigen: Human 14-3-3-zeta N-terminal synthetic peptide
  • Application: WB, IHC
  • Specificity: Does not cross-react with other 14-3-3 proteins
  • Target Entrez Gene ID: 7534

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 0.5 µg/mL
  • Immunohistochemical techniques: 5 µg/mL

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O.

Alternative names

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide, 14-3-3-delta, YWHAZ, YWHAD, KCIP-1




 
 
Products
Cat. # Product Package Size Price # of Units Select
18644A Anti-Human 14-3-3 zeta Protein Rabbit IgG Affinity Purify 10 ug $113.00 *
18644B Anti-Human 14-3-3 zeta Protein Rabbit IgG Affinity Purify 100 ug $499.00
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


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