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Products >  Cell_Biology >  Brain_and_CNS >  Antibodies_14-3-3 >  14-3-3_Sigma_Human

Anti-Human 14-3-3-Sigma Rabbit IgG Antibody

The 14-3-3 family of conserved regulatory proteins is expressed in all eukaryotic cells and are encoded by seven genes in mammals. 14-3-3 proteins bind many types of signaling proteins, including kinases, phosphatases, and transmembrane receptors. More than 100 signaling proteins have been reported as 14-3-3 ligands. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. Elevated amounts of 14-3-3 proteins are found in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease. 14-3-3-sigma, also known as stratifin, epithelial cell marker protein 1, HME1, and YWHAS, is regulated by p53 and plays a role in the G2-M-phase checkpoint.

These products are affinity-purified IgG antibodies that recognize human 14-3-3-sigma protein. The antibodies were raised in rabbit using synthetic peptides, and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of human 14-3-3-sigma protein.

At-A-Glance Documents Images & Data

Features

  • Cat.#: 18642A/B, 18646A/B
  • Target Name: Human 14-3-3-sigma protein
  • Antigen: Human 14-3-3-sigma N-terminal synthetic peptide (for Cat.# 18646A/B) or human 14-3-3-sigma C-terminal synthetic peptide (for Cat.# 18642A/B)
  • Application: WB, IHC
  • Specificity: Does not cross-react with other 14-3-3 proteins
  • Target Entrez Gene ID: 2810

  • Cat.#: 18640A, 18640B
  • Target Name: Human 14-3-3-sigma protein
  • Antigen: Human 14-3-3-sigma synthetic peptide
  • Clone: 69
  • Application: WB, IHC
  • Specificity: Does not cross-react with other 14-3-3 proteins
  • Target Entrez Gene ID: 2810

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 1–3 µg/mL
  • Immunohistochemical techniques: 5 µg/mL

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H20.

Alternative names

stratifin, epithelial cell marker protein 1, HME1, YWHAS




 
 
Products
Cat. # Product Package Size Price # of Units Select
18640A Anti-Human 14-3-3 sigma Protein (69) Rabbit IgG Affinity Purify 10 ug $100.00
18640B Anti-Human 14-3-3 sigma Protein (69) Rabbit IgG Affinity Purify 100 ug $499.00
18646B Anti-Human 14-3-3 sigma Protein (N) Rabbit IgG Affinity Purify 100 ug $499.00
18642A Anti-Human 14-3-3 sigma Protein (C) Rabbit IgG Affinity Purify 10 ug $100.00
18642B Anti-Human 14-3-3 sigma Protein (C) Rabbit IgG Affinity Purify 100 ug $499.00
18646A Anti-Human 14-3-3 sigma Protein (N) Rabbit IgG Affinity Purify 10 ug $100.00
 

 



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