The 14-3-3 family of conserved regulatory proteins is expressed in all eukaryotic cells and are encoded by seven genes in mammals. 14-3-3 proteins bind many types of signaling proteins, including kinases, phosphatases, and transmembrane receptors. More than 100 signaling proteins have been reported as 14-3-3 ligands. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. Elevated amounts of 14-3-3 proteins are found in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease. members of this family play diverse roles, including cell signaling, cell cycle regulation, and regulation of insulin sensitivity.
This product is an affinity-purified IgG antibody that recognizes human 14-3-3 proteins. The antibody was raised in rabbit using a synthetic peptide, and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of human 14-3-3 proteins.