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Products >  Cell_Biology >  Brain_and_CNS >  Antibodies_14-3-3 >  14-3-3_Gamma_Human

Anti-Human 14-3-3-Gamma Protein Antibody

The 14-3-3 family of conserved regulatory proteins is expressed in all eukaryotic cells and are encoded by seven genes in mammals. 14-3-3 proteins bind many types of signaling proteins, including kinases, phosphatases, and transmembrane receptors. More than 100 signaling proteins have been reported as 14-3-3 ligands. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. Elevated amounts of 14-3-3 proteins are found in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease. The 14-3-3-gamma protein is 100% identical to the rat ortholog. It is also known as tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide and YWHAG. Expression of 14-3-3-gamma protein is induced by growth factors in human vascular smooth muscle cells, and is elevated in skeletal and heart muscles. It also interacts with RAF1, a serine/threonine protein kinase, and protein kinase C.

These products are affinity-purified IgG antibodies that recognize human 14-3-3-gamma protein. The antibodies were raised in rabbit (Cat.# 18647) or mouse (Cat.# 10037) using synthetic peptides, and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of human 14-3-3-gamma protein.

At-A-Glance Documents Images & Data

Features

  • Cat.#: 10037A, 10037B
  • Target Name: Human 14-3-3-gamma protein
  • Antigen: Human 14-3-3-gamma C-terminal synthetic peptide
  • Application: WB, IHC
  • Specificity: Does not cross-react with other 14-3-3 proteins
  • Target Entrez Gene ID: 7532

  • Cat.#: 18647A, 18647B
  • Target Name: Human 14-3-3-gamma protein
  • Antigen: Human 14-3-3-gamma C-terminal synthetic peptide
  • Application: WB, IHC
  • Specificity: Does not cross-react with other 14-3-3 proteins
  • Target Entrez Gene ID: 7532

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 1–5 µg/mL
  • Immunohistochemical techniques: 3–5 µg/mL

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O.

Alternative names

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide, YWHAG




 
 
Products
Cat. # Product Package Size Price # of Units Select
10037A Anti-Human 14-3-3 gamma Protein (63A1) Mouse IgG MoAb 10 ug $113.00 *
10037B Anti-Human 14-3-3 gamma Protein (63A1) Mouse IgG MoAb 100 ug $499.00
18647A Anti-Human 14-3-3 gamma Protein Rabbit IgG Affinity Purify 10 ug $113.00 *
18647B Anti-Human 14-3-3 gamma Protein Rabbit IgG Affinity Purify 100 ug $499.00
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


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