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Products >  Cell_Biology >  Brain_and_CNS >  Antibodies_14-3-3 >  14-3-3_Epsilon_Human

Anti-Human 14-3-3-Epsilon Protein Rabbit IgG Antibody

The 14-3-3 family of conserved regulatory proteins is expressed in all eukaryotic cells and are encoded by seven genes in mammals. 14-3-3 proteins bind many types of signaling proteins, including kinases, phosphatases, and transmembrane receptors. More than 100 signaling proteins have been reported as 14-3-3 ligands. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. Elevated amounts of 14-3-3 proteins are found in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease. 14-3-3-epsilon is also known as tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide; mitochondrial import stimulation factor L subunit2, YWHAE, MDCR, MDS, FLJ45465, and KCIP-1. It interacts with CDC25 phosphatases, RAF1, and insulin receptor substrate 1 (IRS1), suggesting diverse functions related to signal transduction, e.g., cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.

This product is an affinity-purified IgG antibody that recognizes human 14-3-3-epsilon protein. The antibody was raised in rabbit using a synthetic peptide, and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of human 14-3-3-epsilon protein.

At-A-Glance Documents Images & Data

Features

  • Cat.#: 18643
  • Target Name: Human 14-3-3-epsilon protein
  • Antigen: Human 14-3-3-epsilon C-terminal synthetic peptide
  • Application: WB, IHC
  • Specificity: Does not cross-react with other 14-3-3 proteins
  • Target Entrez Gene ID: 7531

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 1–5 µg/mL
  • Immunohistochemical techniques: 2–3 µg/mL

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O.

Alternative names

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide; mitochondrial import stimulation factor L subunit2, YWHAE, MDCR, MDS, FLJ45465, KCIP-1




 
 
Products
Cat. # Product Package Size Price # of Units Select
18643A Anti-Human 14-3-3 epsilon Protein Rabbit IgG Affinity Purify 10 ug $100.00
18643B Anti-Human 14-3-3 epsilon Protein Rabbit IgG Affinity Purify 100 ug $499.00
 

 



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