TRAP and ALP Double Stain Kit

Bone metabolism is mutually balanced between osteoblast-mediated bone formation and osteoclast-mediated bone resorption. Tartrate-resistant acid phosphatase (TRAP, also known as TRACP) and alkaline phosphatase (ALP) staining enables effective discrimination between osteoblast and osteoclast bone cells. Takara's TRAP and ALP Double Stain Kit combines the chromogenic substrates for alkaline phosphatase, an osteoblast enzyme marker, and tartrate-resistant acid phosphatase, an osteoclast enzyme marker, with a nuclear staining reagent. This enables osteoblast and osteoclast detection due to the simultaneous cell staining for both alkaline and acid phosphatase activities. Detection of both enzyme markers provides a means to study the differentiation of bone cells. The kit substrates are provided as premixed reagents for ease of use. Sufficient reagent is supplied for staining approximately 5 culture plates (24 well format).