TRACP and ALP Double Stain Kit

The TRACP and ALP Double Stain Kit allows differential staining of osteoblast and osteoclast cells (bone-related cells). With this kit, chromogenic substrates for alkaline phosphatase, an enzyme marker of osteoblasts, and tartrate-resistant acid phosphatase, an enzyme marker of osteoclasts, are combined with a reagent for nuclear staining that provides visualization of multinucleated osteoclasts. Both acid and alkaline phosphatase activities in the cells can be stained simultaneously for comparison. Bone metabolism is mutually balanced between bone formation by osteoblasts and bone absorbsion by osteoclasts. Simultaneous detection of both enzyme markers provides a means to study the differentiation of bone-related cells. The kit substrates are provided as premixed reagents for ease of preparation. Sufficient reagent is supplied for staining approximately 5 culture plates (24 well size).