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Products >  Cell_Biology >  Bone_Research >  Reagents >  TRACP_and_ALP_Assay_Kit

TRACP and ALP Assay Kit

TRACP & ALP Assay Kit allows for simultaneous detection of 2 enzymes which are involved in bone metabolism. TRACP is an osteoclast enzyme marker and ALP an osteoblast enzyme marker. TRACP & ALP Assay Kit has been designed for simple and quick detection of ACP (Acid phosphatase) and ALP (Alkaline phosphatase) through the use of pNPP (p -nitro-phenyl phosphate) substrate. The addition of tartaric acid into the ACP assay, allows for the detection of TRACP (tartrate-resistant acid phosphatase) activity. Since this kit utilizes an aqueous substrate, it enables quick activity quantification by measuring the absorbance of the reactant. In addition to this kit, TRACP & ALP double-staining Kit (Cat. #MK300) is also available using a non-soluble substrate. The appropriate kit can be selected depending on assay interest.

At-A-Glance Documents

Features

  • Simultaneous assay of multiple samples
  • Available for measurement of two enzyme activities in one sample

Applications

  • Detection of Acid Phosphatase (ACP) and Alkaline Phosphatase (ALP)

Kit components (for 500 reactions)

1. pNPP (p-nitro-phenyl phosphate) substrate [pNPP substrate] 24 mg×5
2. Extraction solution 11 ml×2
3. Sodium tartrate solution 4 ml
4. Buffer for ACP 30 ml
5. Buffer for ALP 30 ml
6. Microplate (96-well) 1 plate

Storage

4°C




 
 
Products
Cat. # Product Package Size Price
MK301 TRACP and ALP Assay Kit 96 Assays $347.00
 

 



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