Generally, low molecular weight peptides and synthetic peptides do not absorb spontaneously to 96 well microtiter plates. This kit is designed to allow optimum coating of peptides on plates effectively and easily. The Peptide Coating Kit includes buffer, 96 well microplates and blocking solution specialized for subsequent ELISA.
Principle
The free carboxyl group of the peptide to be used for coating should be covalently linked with EDC (Coupling Reagent), and subsequently linked with an amino group that is attached on the surface of the microplate wells. The result is peptide directly coated on the surface of the microplate through a peptide bond.
