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Products >  Cell_Biology >  Bone_Research >  EIA_Kits >  Undercarboxylated_Glu-OC_EIA_Kit

Undercarboxylated Osteocalcin (Glu-OC) EIA Kit

The Undercarboxylated Osteocalcin (Glu-OC) EIA Kit is a 96-well in vitro enzyme immunoassay kit intended for the quantitative determination of human undercarboxylated osteocalcin (Glu-OC) in serum, plasma, urine, cultured cell extract, cell culture supernatant and other biological fluids. It is a solid-phase sandwich human osteocalcin ELISA that utilizes two mouse monoclonal Glu-OC antibodies, one of which is coated onto the plate and the other of which is peroxidase-labeled for the capture and detection of undercarboxylated osteocalcin. The assay involves two steps: In the first step, samples are incubated on the antibody-coated microtiter plate; during the second step, the plate is washed and incubated with the peroxidase-labeled Glu-OC antibody. A substrate is added and the reaction between the peroxidase and the substrate (H2O2, TMBZ) results in color development. The amount of Glu-OC is determined by measuring absorbance using an EIA plate reader. Accurate Glu-OC sample concentrations can be determined by comparing their specific absorbances with the absorbance obtained from a standard curve (using the supplied kit standard).

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Features

  • Sandwich ELISA for highly sensitive quantitation of human Glu-OC

 

Applications

  • Quantitative measurement of human, bovine, rabbit, sheep, pig, goat, and monkey undercarboxylated osteocalcin.
  • Undercarboxylated Osteocalcin (Glu-OC) EIA

Components

Antibody coated microtiter plate1 plate (8 wells x 12 strips)
Antibody-peroxidase conjugate (lyophilized)1 vial (for 1 x 11 mL)
Standard1 vial (8 ng)
Sample diluent2 vials (2 x 11 mL)
Substrate solution1 vial (1 x 12 mL)

Storage

2–8°C

Performance Characteristics

  • Range of assay: 0.25-8 ng/mL
  • Assay sensitivity: 0.5 ng/mL
  • Assay duration: 3.5 hours
  • Specificity: Human Glu-OC.
  • Species cross-reactivity: Reactivity with bovine, rabbit, sheep, pig and goat Glu-OC. No reactivity with mouse and rat Glu-OC. Applications for other species have not been tested.
  • Test specimen type: Serum, plasma, urine, culture supernatant or cell extract.
  • Sample volume per well: 100 µL



 
 
Products
Cat. # Product Contents Size Price License Units Select
MK118 Undercarboxylated Osteocalcin (Glu-OC) EIA Kit 96 Rxns $713.00 License Statements
 

 

 
 

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