In the first step, samples are incubated in the antibody-coated microtiter plate. During the second step, the plate is washed and incubated with the POD-labeled Glu-OC antibody. A substrate is added, and the reaction between POD and the substrate (H2O2, TMBZ) results in a color development. The amount of sample Glu-OC is determined by measuring absorbance using an EIA plate reader. Accurate Glu-OC sample concentrations can be determined by comparing their specific absorbances with the absorbance obtained for the Standard plotted on a standard curve.
