Procollagen Type I C-Peptide (PIP) EIA Kit

The Procollagen Type I C-Peptide (PIP) EIA Kit is a 96 well in vitro enzyme immunoassay for the quantitative determination of human, bovine, or canine PIP in plasma, serum, cultured cell extracts, cell culture supernatants, and other biological fluids.It is a solid phase EIA based upon a sandwich design that utilizes two mouse monoclonal PIP antibodies (one of which is coated on the plate, and the other is POD-labeled) for detection of PIP using a one step incubation method. Samples and POD-anti-PIP are simultaneously added to the wells of the plate and incubated. During incubation, PIP becomes bound to the antibody coating the plate, and is then tagged by POD-anti-PIP on its opposite side. A substrate is added, and the reaction between POD and the substrate (H₂O₂, TMBZ) results in a color development. The amount of sample PIP is determined by measuring absorbance using an EIA plate reader. Accurate PIP sample concentrations can be determined by comparing their specific absorbances with the absorbance obtained for the Standards plotted on a standard curve.