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Products >  Cell_Biology >  Bone_Research >  EIA_Kits >  Osteocalcin_EIA_Kits_Pig

Bone Osteogenesis and Remodeling: Osteocalcin EIA Kits (Pig)

The Pig Gla-Osteocalcin EIA Kit (Cat. #MK139) is a quantitative assay that measures bone osteogenesis via the specific and highly sensitive detection of porcine cOC (an active osteocalcin that exhibits a propensity towards osseointegration). The capture molecule is a plate-bound solid-phase monoclonal antibody that specifically recognizes the osteocalcin Gla residue at position 17. This monoclonal antibody is paired with a labeled monoclonal antibody for porcine osteocalcin detection.

The Pig Glu-Osteocalcin EIA Kit (Cat. #MK149) is a quantitative assay that measures bone osteogenesis via the specific and highly sensitive detection of ucOC released from porcine bone tissues by osteoclasts or inactive ucOC produced by osteoblasts. The capture molecule is a plate-bound solid-phase monoclonal antibody that specifically recognizes the ostecalcin Glu residues at positions 21 and 24. This monoclonal antibody is paired with a labeled monoclonal antibody for porcine osteocalcin detection.

Quantitative Osteocalcin Analysis

Bone osteogenesis is often detected through osteocalcin (OC), a bone-specific protein that is synthesized only by bone-forming osteoblasts. Because of osteocalcin's exclusivity to osteoblasts, it is often used as a marker specific to bone osteogenesis. Osteocalcin is comprised of 49 amino acids, including 2-3 γ-carboxyglutamate residues (Gla), and has a molecular weight of approximately 5.9 kDa. It is known to be a vitamin K-dependent, calcium-binding non-collagenous protein. It is also a hormone that plays an important role in glucose metabolism. While carboxylated, osteocalcin (cOC) is active in bone metabolism; undercarboxylated osteocalcin (ucOC) is active in glucose metabolism. The Pig Gla-Osteocalcin EIA Kit (Cat. #MK139) and the Pig Glu-Osteocalcin EIA Kit (Cat. #MK149) may be used to monitor cOC and ucOC, respectively, during bone osteogenesis and remodeling. The pig osteocalcin ELISA Kit facilitates the relative evaluation of Gla/Glu-osteocalcins.

Animal Models for Bone Remodeling

During bone osteogenesis, the summation of dynamic processes occurring in growing young animals is termed "modeling" (new construction). In mature animals, bone morphology undergoes no apparent changes and appears to be stable, although a certain percentage of the bone is being constantly replaced. This process is called "remodeling" (reconstruction). Efficacy assessments of osteoporosis drugs require the use of two animal model types during the evaluation of bone modeling and remodeling: Rodents, such as mouse and rat, are used to evaluate modeling, while monkeys and miniature pigs are used to evaluate remodeling. Thus, animal models are essential for the development and assessment of potential drug targets.

At-A-Glance Documents Images & Data Resources

Features

  • Quantitative detection of bone osteogenesis associated osteocalcin produced in porcine serum, plasma, ascite fluid, urine and cell culture supernatant.
  • Pig Gla-Osteocalcin EIA kit is intended for the detection of porcine cOC.
  • Pig Glu-Osteocalcin EIA kit is intended for the detection of porcine ucOC.

Applications

  • Bone osteogenesis and remodeling studies
  • Specific detection of pig Gla-OC or Glu-OC by ELISA
    • Components

      1.Antibody Coated Microtiter plate1 plate (8 wells × 12 strips)
      2.Antibody - Peroxidase Conjugate1 vial (for 11 ml)
      3.Standard1 vial (for 1 ml)
      4.Sample Diluent2 vials (11 ml × 2)
      5.Substrate Solution1 vial (12 ml)

      Storage

      4°C

      Performance

      Pig Gla-Osteocalcin EIA Kit (Cat. #MK139)

      Range of assay:1.0 - 64.0 ng/ml
      Sensitivity:1.0 ng/ml
      Specificity:Porcine Gla-OC
      Cross-reactivity:bovine*1 and rat*2
      Assay duration:2.5 hours
      Test specimen type:porcine serum, plasma, ascite fluid, urine and cell culture supernatant
      Specimen volume required:100 µl/assay well

      Pig Glu-Osteocalcin EIA Kit (Cat. #MK149)

      Range of assay:0.5 - 32.0 ng/ml
      Sensitivity:0.5 ng/ml
      Specificity:Porcine Glu-OC
      Cross-reactivity:bovine*1 and rat*2
      Assay duration:2.5 hours
      Test specimen type:porcine serum, plasma, ascite fluid, urine and cell culture supernatant
      Specimen volume required:100 µl/assay

      *1:These products weakly cross-react with bovine antigens; any bovine serum supplemented medium in a cultured sample may interfere with the assay. For best results, consider switching to a serum-free medium.
      *2:These products weakly cross-react with rat antigens, resulting in low sensitivity. Therefore, they are unsuitable for assaying samples of rat origin. We recommend Rat Gla-Osteocalcin High Sensitive EIA Kit (Cat. #MK126) or Rat Glu-Osteocalcin High Sensitive EIA Kit (Cat. #MK146) for assaying rat samples.




       
       
      Products
      Cat. # Product Package Size Price # of Units Select
      MK139 Pig Gla-Osteocalcin EIA Kit 96 Rxns $653.00
      MK149 Pig Glu-Osteocalcin EIA Kit 96 Rxns $653.00
       

       



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