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Products >  Cell_Biology >  Bone_Research >  EIA_Kits >  Gla-Osteocalcin_High_Sensitive_EIA_Kit-Rat

Bone Formation and Resorption: Rat Osteocalcin High Sensitive EIA Kits

The Rat Gla-Osteocalcin High Sensitive EIA Kit (Cat #MK126) is a 96-well format sandwich-type EIA that takes advantage of a C-terminal rat osteocalcin monoclonal antibody to capture osteocalcin (OC) in rat biological samples. This antibody has minimal cross-reactivity to bovine, human and rabbit osteocalcin. An enzyme-labeled antibody (Gla-OC4-30) specific to Gla-OC is used as the detection antibody, allowing this kit to detect Gla-osteocalcin with very high sensitivity. As such, this EIA kit is sensitive enough to pick up even minute traces of rat Gla-osteocalcin in cell supernatants supplemented with fetal calf serum.

The Rat Glu-Osteocalcin High Sensitive EIA Kit (Cat.# MK146) is a 96-well format sandwich-type EIA that uses a C-terminal rat osteocalcin monoclonal antibody to capture ostecalcin (OC). The kit's detection antibody specifically recognizes the glutamate residues present at positions 21 and 24 of the protein, making highly sensitive yet reproducible measurements of low level Glu-OC possible. Moreover, because both the Rat Gla-Osteocalcin High Sensitive EIA Kit (Cat.#MK126) and the Rat Glu-Osteocalcin High Sensitive EIA Kit (Cat.# MK146) contain the same capture antibody, both kits may be used for Gla/Glu detection (Cat.# MK147), making simultaneous monitoring of bone formation and resorption possible.

Bone formation and resorption requires the action of osteoblasts, which produce osteocalcin exclusively. Osteocalcin is typically produced with all three of its glutamate residues carboxylated (Gla-OC), affording the protein the ability to form a calcium pocket and bind bone matrix. The carboxylated glutamate residues are at positions 17, 21 and 24 of the protein. During bone metabolism, osteocalcin is released from bone matrix through the action of various enzymes, including one produced by osteoclasts. Most of the three glutamate residues are decarboxylated on osteocalcin (Glu-OC) when it is released into bloodstream from bone. Thus, osteocalcin is present in blood in both its Gla and Glu forms.

Osteocalcin, which contains 2-3 gamma-carboxyglutamate (Gla) residues and has a molecular weight of approximately 5.9 kDa, is known as a vitamin K-dependent calcium-binding non-collagenous protein. Produced only by osteoblasts, osteocalcin has been used as a unique marker of bone formation and resorption. The rat osteocalcin protein consists of 50 amino acids while human, bovine, rabbit and other species express an osteocalcin protein containing 49 amino acids. Osteocalcin is also a hormone that plays a role in insulin regulation and male fertility. Meanwhile, the undercarboxylated form of osteocalcin (ucOC) is responsible for glucose metabolism. The Gla-osteocalcin and Glu-osteocalcin ELISA kits enable researchers to study the roles of both carboxylated and undercarboxylated osteocalcin in metabolic disease, male fertility and bone formation and resorption.

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Features

  • Highly sensitive detection of rat osteocalcin in cell supernatants supplemented with fetal calf serum
  • Glu-Osteocalcin: for detection of undercarboxylated form of rat osteocalcin
  • Gla-Osteocalcin: for detection of the carboxylated form of rat osteocalcin

Applications

  • Bone formation and resorption analysis
  • Quantitative determination of Gla-osteocalcin (rat Gla-OC) and/or Glu-osteocalcin (rat Glu-OC) in rat-derived biological samples
  • Gla-Osteocalcin and Glu-Osteocalcin ELISA

Kit Components

MK126 and MK146

Antibody Coated Microtiterplate

1 plate (8 wells × 12 strips)
Antibody - peroxidase conjugate1 vial (for 11 mL)
Standard1 vial (for 1 mL)
Sample Diluent2 vials (11 mL × 2)
Substrate Solution1 vial (12 mL)

MK147 
Contains 1 each of MK126 and MK146

Performance Characteristics

Rat Gla-Osteocalcin High Sensitive EIA Kit:

Range of assay:0.25–16 ng/mL
Sensitivity:0.25 ng/mL
Assay duration:2.5 hours
Specificity:Rat Gla-OC
Test specimen type:Rat serum, ascites, cell culture supernatant and cell extract
Specimen volume required:100 µL/assay well

Rat Glu-Ostecocalin High Sensitive EIA Kit:

Range of assay:0.125 - 8 ng/ml
Sensitivity:0.125 ng/ml
Assay duration:2.5 hours 
Specificity:Rat Glu-OC
Test specimen type:Rat serum, ascites, cell culture supernatant, and cell extract
Specimen volume required:100 µl/assay well

References

  1. Price, P.A. et al. (1980) J. Biol. Chem. 255(7):2938-2942.
  2. Patterson-Allen, P. et al. (1982) Anal. Biochem. 120:1-7.



 
 
Products
Cat. # Product Contents Size Price Units Select
MK147 Gla/Glu Osteocalcin High Sensitive EIA Set (Rat) Each $1,044.00
MK126 Rat Gla-Osteocalcin High Sensitive EIA Kit 96 Rxns $653.00
MK146 Rat Glu-Osteocalcin High Sensitive EIA Kit 96 Rxns $653.00
 

 

Recommended Products
Cat. # Product Contents Size Price License Units Select
MK111 Gla-Type Osteocalcin (Gla-OC) EIA Kit 96 Assays $713.00  
MK118 Undercarboxylated Osteocalcin (Glu-OC) EIA Kit 96 Rxns $713.00 License Statements
 

 

 
 

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