This Takara Bio kit for osteocalcin ELISA detects human osteocalcin. Osteocalcin, also known as bone gamma-carboxyglutamate protein, is a vitamin K and D-dependent, calcium-binding, non-collagenous protein. It has a molecular weight of approximately 5.9 kDa and contains 2-3 gamma-carboxyglutamate residues (Gla). Osteocalcin is produced only by osteoblasts and their dental counterpart, odontoblasts, and is an indicator of bone metabolism. The carboxylated form of osteocalcin is an indicator of bone formation, while its undercarboxylated counterpart is involved in glucose metabolsim. Osteocalcin is also a hormone that regulates glucose and fat deposition as well as male fertility.
The Gla-Osteocalcin High Sensitive EIA Kit (Human) kit is designed to specifically differentiate human from bovine osteocalcin using a human osteocalcin-specific coated plate. This coated plate contains a monoclonal antibody that distinguishes human from bovine osteocalcin via their amino acid differences (at positions 3 and 4) at the N-terminus. Such discrimination makes it possible to accurately distinguish human versus bovine osteocalcin. Using a human antigen-specific monoclonal antibody as the capture antibody greatly improves experimental linearity with human blood samples.
The specificity of this human osteocalcin ELISA also enables direct assay of human osteocalcin in osteoclast culture supernatant, differentiated bone marrow osteoblasts and mesenchymal stem cells cultured in bovine serum-containing medium. The detection antibody in this kit specifically recognizes the osteogenic marker gamma-carboxyglutamate (Gla) at position 17, allowing direct measurement of Gla-type (active type) osteocalcin in bone (primarily hydroxyapatite) matrix. This kit may be used to measure osteocalcin in human blood.