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Products >  Cell_Biology >  Bone_Research >  Antibodies >  Osteonectin-SPARC_Monoclonal

Monoclonal Anti-Osteonectin (SPARC) Antibody (Clone ON1-1 and OSN4-2)

Osteonectin is a 43 kDa non-collagenous glycoprotein found in the bone extracellular matrix. Monoclonal Anti-Ostenectin/SPARC (Clone ON-1 and Clone OSN4-2) antibody was raised against bone-derived bovine osteonectin (ON1-1) and human platelet-derived osteonectin (OSN4-2). The osteonectin antibody reacts with activated (but not non-activated) platelets, facilitating thrombin-stimulated platelet detection.

At-A-Glance Documents Images & Data Resources

Applications

  • Western blotting analysis under reducing or non-reducing conditions
  • Immunohistochemical analysis of frozen or paraffin-embedded tissue sections 
  • Flow cytometry
  • Detection of activated platelets

Source

CloneCat.#ImmunogenImmunized hostSubclassAntibody titer
OSN4-2M124human platelet osteonectinBALB/c mouseIgG11:4,000
ON1-1M125bovine bone osteonectinBALB/c mouseIgG11:4,000

Storage

Lyophilized antibody is stable at 4°C for up to 2 years. The reconstituted solution can be stored at 4°C for 6 months and at –20°C for up to 1 year.

Notes

For research use only. Not intended for diagnostic or therapeutic use.

Form

Lyophilized

Reconstitution

Dissolve the lyophilized antibody in 50 µl of distilled water (final concentration: 2.0 mg/ml).

References

1. Funk, S.E. & Sage, E.H. (1991) Proc. Natl. Acad. Sci. USA 88:2648-2652. 
2. Termine, J.D. et al. (1981) Cell 26:99-105. 
3. Breton-Gorius, J. et al. (1992) Blood 79:936-941. 
4. Kamihagi, K. et al. (1994) Biochem. Biophys. Res. Comm. 200:423-428. 
5. Nakamura, S. et al. (1996) Arthritis and Rheumatism 39:539-551.

Specificity

  • These products can react with both bone-derived and platelet-derived osteonectin.
  • These products react with thrombin-stimulated but not non-activated platelets (flow cytometry).
  • These products react with human and bovine osteonectin with the same efficiency (Western blotting).
  • These products cross-react with rabbit and porcine osteonectin (ELISA).



 
 
Products
Cat. # Product Package Size Price # of Units Select
M125 Monoclonal Anti-Osteonectin/SPARC (Clone ON1-1) 0.1 mg $357.00
M124 Monoclonal Anti-Osteonectin/SPARC (Clone OSN4-2) 0.1 mg $357.00
 

 



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