Applications - Western blotting analysis under reducing or non-reducing conditions
- Histology on frozen tissue sections. Clone ON1-1 can also be used for paraffin embedded tissue sections.
- Flow cytometry
Source| Clone | Cat.# | Immunogen | Immunized host | Subclass | Antibody titer | | OSN4-2 | M124 | human platelet osteonectin | BALB/c mouse | IgG1 | 1:4,000 | | ON1-1 | M125 | bovine bone osteonectin | BALB/c mouse | IgG1 | 1:4,000 |
StorageLyophilized antibody is stable at 4°C for 2 years. The reconstituted solution can be stored at 4°C for 6 months, and at –20°C for 1 year.
NotesFor research use only. Not for use in diagnostic or therapeutic procedures.
FormLyophilized ReconstitutionDissolve the lyophilized antibody in 50 µl of distilled water (final concentration: 2.0 mg/ml).
Specificity - These products can react with both bone-derived and platelet-derived osteonectin.
- These products react with thrombin-stimulated platelet and do not react with non-activated platelet (flow cytometry) .
- These products react with human and bovine osteonectin at the same efficiency (Western blotting).
- These products cross-react with rabbit and porcine antigen (ELISA).
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