Applications M185- Osteocalcin immunohistochemistry using formalin fixed paraffin embedded tissues (antigen retrieval improved with proteinase K treatment)
- Cannot be used for Western blot analysis
M186- Osteocalcin immunohistochemistry using formalin fixed paraffin embedded tissues (antigen retrieval improved with proteinase K treatment)
- Western blot analysis to detect full-length rat osteocalcin under reducing and non-reducing conditions
M187- Western blot analysis using rat bone extract under reducing and non-reducing conditions
- Cannot be used for osteocalcin immunohistochemistry
Source| Clone | Cat.# | Immunogen | Immunized host | Subclass | Antibody titer | | D-8G | M185 | KLH-conjugated rat osteocalcin peptide | BALB/c mouse | IgG1 | 1:1,000 | | 6-7H | M186 | | 9-12H | M187 |
Storage 4°C
The stock solution (2.0 mg/ml) should be stored in aliquots at -20°C for 1 year or at 4°C for 6 months. Avoid repeated freeze-thaws.
Specificity| Clone | Cat.# | Epitope | Crossreactivity | No reactivity | | D-8G | M185 | rat osteocalcin amino acid residues 1-25: YLNNGLGAPAPYPDPLEPHREVCEL | rat, bovine, pig, goat, sheep, and donkey | human | | 6-7H | M186 | bovine | | 9-12H | M187 | rat osteocalcin amino acid residues 38-50: FQDAYKRIYGTTV | rat | human, bovine |
FormLyophilized ReconstitutionDissolve lyophilized antibody in 50 µl of distilled water (final concentration: 2.0 mg/ml).
|