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Products >  Cell_Biology >  Bone_Research >  Antibodies >  Osteocalcin-Rat_Monoclonal

Monoclonal Anti-Rat Osteocalcin (Clone D-8G, Clone 6-7H, Clone 9-12H)

Monoclonal Anti-Rat Osteocalcin (Clone D-8G, Clone 6-7H, Clone 9-12H) antibodies selectively detect rat osteocalcin. Cat. # M185 and Cat. # M186 may be used for osteocalcin immunohistochemistry with formalin-fixed and paraffin-embedded tissues, while Cat. # M186 and Cat. # M187 may be used for Western blot analysis.

At-A-Glance Documents Images & Data Resources

Applications

M185

  • Osteocalcin immunohistochemistry using formalin fixed paraffin embedded tissues (antigen retrieval improved with proteinase K treatment)
  • Cannot be used for Western blot analysis

M186

  • Osteocalcin immunohistochemistry using formalin fixed paraffin embedded tissues (antigen retrieval improved with proteinase K treatment)
  • Western blot analysis to detect full-length rat osteocalcin under reducing and non-reducing conditions

M187

  • Western blot analysis using rat bone extract under reducing and non-reducing conditions
  • Cannot be used for osteocalcin immunohistochemistry

Source

CloneCat.#ImmunogenImmunized hostSubclassAntibody titer
D-8GM185

KLH-conjugated rat osteocalcin peptide

BALB/c mouseIgG11:1,000
6-7HM186
9-12HM187

Storage

4°C

The stock solution (2.0 mg/ml) should be stored in aliquots at -20°C for 1 year or at 4°C for 6 months. Avoid repeated freeze-thaws.

Specificity

CloneCat.#EpitopeCrossreactivityNo reactivity
D-8GM185rat osteocalcin amino acid residues 1-25: YLNNGLGAPAPYPDPLEPHREVCELrat, bovine, pig, goat, sheep, and donkeyhuman
6-7HM186bovine
9-12HM187rat osteocalcin amino acid residues 38-50: FQDAYKRIYGTTVrathuman, bovine

Form

Lyophilized

Reconstitution

Dissolve lyophilized antibody in 50 µl of distilled water (final concentration: 2.0 mg/ml).




 
 
Products
Cat. # Product Package Size Price # of Units Select
M186 Monoclonal Anti-Rat Osteocalcin (Clone 6-7H) 0.1 mg $357.00
M187 Monoclonal Anti-Rat Osteocalcin (Clone 9-12H) 0.1 mg $357.00
M185 Monoclonal Anti-Rat Osteocalcin (Clone D-8G) 0.1 mg $357.00
 

 


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