Applications M185- Immunohistochemical detection of osteocalcin on paraffin embedded tissue fixed formalin (Antigen retrieval: Proteinase K treatment)
- Cannot be used for western blotting analysis
M186- Immunohistochemical detection of osteocalcin on paraffin embedded tissue fixed formalin (Antigen retrieval: Proteinase K treatment)
- Western blotting analysis using synthetic peptide of rat osteocalcin full sequence under reducing and non-reducing conditions
M187- Western blotting analysis using rat bone extract under reducing and non-reducing conditions
- It cannot be used for immunohistochemical detection
Source| Clone | Cat.# | Immunogen | Immunized host | Subclass | Antibody titer | | D-8G | M185 | peptide of rat osteocalcin-KLH conjugate | BALB/c mouse | IgG1 | 1:1,000 | | 6-7H | M186 | | 9-12H | M187 |
Storage 4°C
The stock solution (2.0 mg/ml) should be stored in aliquots at -20°C for 1 year, or at 4°C for 6 months. Avoid repeated freeze-thaws.
Specificity| Clone | Cat.# | Epitope | React with | Not react with | | D-8G | M185 | amino acid sequence of rat osteocalcin (residue 1-25) | rat, bovine, pig, goat, sheep, and donkey | human | | 6-7H | M186 | bovine | | 9-12H | M187 | amino acid sequence of rat osteocalcin (residue 38-50) | rat | human, bovine |
FormLyophilized ReconstitutionDissolve the lyophilized antibody in 50 µl of distilled water (final concentration: 2.0 mg/ml).
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