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Products >  Cell_Biology >  Bone_Research >  Antibodies >  Osteocalcin-Mouse_Monoclonal

Monoclonal Anti-Mouse Osteocalcin (Clone R21C-01A)

Monoclonal Anti-Mouse Osteocalcin (Clone R21C-01A) was obtained by fusing mouse myeloma cell-line P3U1 with the spleen cells of Sprague Dawley rats following their immunization with mouse osteocalcin peptide (amino acids 25-46) conjugated to keyhole limpet hemocyanin (KLH). The osteocalcin antibody was harvested from severe combined immunodeficiency (SCID) mouse ascitic fluid.

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Applications

  • Osteocalcin immunohistochemistry
  • Immunohistochemical detection of osteocalcin on formalin fixed paraffin-embedded mouse tissue (treated with proteinase K for improved antigen retrieval)
  • Western blot analysis using mouse bone extract under reducing or non-reducing conditions

Source

CloneCat.#ImmunogenImmunized hostSubclassAntibody titer
R21C-01AM188mouse osteocalcin peptide (amino acids 25-46: CDELSDQYGLKTAYKRIYGITI) conjugated to KLHSD/ratRat IgG2a1:1,000

Storage

Lyophilized antibody is stable at 4°C for 2 years. The stock solution (2.0 mg/mL) with Reconstitution Solution (0.1% sodium azide in water) can be stored in aliquots at –20°C for up to 1 year or at 4°C for up to 6 months. Avoid repeated freeze-thaw cycles. The diluted antibody should not be stored.

Specificity

Clone: R21C-01A
Specificity: Mouse osteocalcin amino acid sequence 25-46: CDELSDQYGLKTAYKRIYGITI
Subclass: Rat IgG2a

  • This product specifically reacts with mouse osteocalcin.
  • This product does not react with bovine, rat and human osteocalcin.

Form

Lyophilized

Reconstitution

Dissolve the lyophilized antibody in 50 µl of distilled water (final concentration: 2.0 mg/ml).


Working Concentration

  • 5-10 µg/mL for colorimetric immunohistochemical staining
  • 5-10 µg/mL for colorimetric Western blot



 
 
Products
Offers Cat. # Product Contents Size Price Units Select
M188 Monoclonal Anti-Mouse Osteocalcin (Clone R21C-01A) 0.1 mg $357.00
 

 

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