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Products >  Cell_Biology >  Bone_Research >  Antibodies >  Osteocalcin-Human_Monoclonal

Monoclonal Anti-Human Osteocalcin (Clone GluOC4-5 and Clone 5-12H)

Takara Bio offers antibodies for detection of human osteocalcin. Monoclonal Anti-Human Undercarboxylated Osteocalcin (Clone GluOC4-5) was raised against human osteocalcin peptide and specifically recognizes human osteocalcin with decarboxylated glutamic acid residues. This antibody does not recognize Gla-type osteocalcin. Monoclonal Anti-Human Osteocalcin (Clone 5-12H) was obtained by fusing mouse myeloma cell-line P3U1 with C57BL6 mouse spleen cells following immunization with keyhole limpet hemocyanin (KLH)-conjugated to human osteocalcin peptide (amino acid residues 1-6). This monoclonal antibody was harvested from severe combined immunodeficiency (SCID) mouse ascitic fluid, and is useful for detection of full-length human osteocalcin.

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Applications

  • Clone 5-12H:
    Paraffin-embedded tissue histological analysis
    Western blot analysis under reducing or non-reducing conditions

  • Clone GluOC4-5:
    Paraffin-embedded tissue (acetone, methyl benzoate, xylene (AMeX) modified method fixed) histological analysis
    Western blot analysis under reducing or non-reducing conditions

Source

CloneCat.#ImmunogenImmunized hostSubclassAntibody titer
GluOC4-5M171human osteocalcin (aa 14-30) peptide conjugated to KLHBALB/c mouseIgG11:500
5-12HM184human osteocalcin (aa 1-6) peptide conjugated to KLHC57BL6 mouseIgG2b1:1000

Storage

Lyophilized antibody is stable at 4°C for up to 2 years. The reconstituted solution can be stored at 4°C for 6 months and at –20°C for up to 1 year.

Form

Lyophilized

Reconstitution

Dissolve lyophilized antibody in 50 µl of distilled water (final concentration: 2.0 mg/mL).

References

  1. Vergnaud, P. et al. (1997) J. Cln. Endocrinol. Metab. 82:719-724.

Specificity

CloneCat.#EpitopeReacts withDoes not react with
GluOC4-5M171Human OC with decarboxylated glutamic acid at residue 21 and 24bovine, rabbit, rat, dog, guinea pig, porcine, sheep and goat OC with decarboxylated Glu-21 and Glu-24Gla-type osteocalcin
5-12HM184amino acid sequence of human osteocalcin (residues 1-6)human bovine, rat, guinea pig, porcine, horse, pigeon and goose




 
 
Products
Offers Cat. # Product Contents Size Price Units Select
M184 Monoclonal Anti-Human Osteocalcin (Clone 5-12H) 0.1 mg $357.00
M171 Monoclonal Anti-Human Undercarboxylated Osteocalcin (Clone GluOC4-5) 0.1 mg $357.00
 

 

Recommended Products
Offers Cat. # Product Contents Size Price License Units Select
MK111 Gla-Type Osteocalcin (Gla-OC) EIA Kit 96 Assays $713.00  
MK118 Undercarboxylated Osteocalcin (Glu-OC) EIA Kit 96 Rxns $713.00 License Statements
M041 Monoclonal Anti-Bovine Osteocalcin (Clone OC4-30) 0.1 mg $357.00  
 

 

 
 

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