Takara
login | register | order now | view cart  
Products
Cell Biology
Allergy and Immunology
Apoptosis Kits and Reagents
Bone Research
Cell Adhesion and Extracellular Matrix
Cell Proliferation and Viability
Epigenetics
Miscellaneous
Signal Transduction


Products >  Cell_Biology >  Bone_Research >  Antibodies >  Osteocalcin-Human_Monoclonal

Osteocalcin (Human), Monoclonal

(M171) This clone was raised against human osteocalcin peptide. It specifically recognizes human osteocalcin with decarboxylated glutamic acid residues, and does not recognize Gla-type osteocalcin.

(M184) This monoclonal antibody was obtained by fusing the mouse myeloma cell-line P3U1 with spleen cells of C57BL6 mouse after immunization with KLH-conjugated peptide from human osteocalcin sequence (amino acids 1-6 YLYQWL). The monoclonal antibody was harvested from scid mouse ascitic fluid.

At-A-Glance Documents

Applications

  • Clone 5-12H
    Histology on paraffin embedded tissue
    Western blotting analysis under reducing or non-reducing conditions
  • GluOC4-5
    Histology on paraffin embedded tissue fixed by AMeX modified method
    Western blotting analysis under reducing or non-reducing conditions

Source

CloneCat.#ImmunogenImmunized hostSubclassAntibody titer
5-12HM184peptide of human osteocalcin (1-6)-KLH conjugateC57BL6 mouseIgG2b1:1000
GluOC4-5M171peptide of human osteocalcin (14-30)-KLH conjugateBALB/c mouseIgG11:500

Storage

Lyophilized antibody is stable at 4°C for 2 years. The reconstituted solution can be stored at 4°C for 6 months, and at –20°C for 1 year.

Form

Lyophilized

Reconstitution

Dissolve the lyophilized antibody in 50 µl of distilled water (final concentration: 2.0 mg/mL).

References

  1. Vergnaud, P. et al. (1997) J. Cln. Endocrinol. Metab. 82, 719-724.

Specificity

CloneCat.#EpitopeReact withNot react with
5-12HM184amino acid sequence of human osteocalcin (residue 1-6)humanbovine, rat, guinea pig, porcine, horse, pigeon and goose
GluOC4-5M171glutamic acid (decarboxylated) sequence of human osteocalcin (residue 21 and 24)bovine, rabbit, rat, dog, guinea pig, porcine, sheep and goat osteocalcin.Gla-type osteocalcin


 
 
Products
Cat. # Product Package Size Price
M184 Monoclonal Anti-Human Osteocalcin (Clone 5-12H) 0.1 mg $350.00
M171 Monoclonal Anti-Human Undercarboxylated Osteocalcin (Clone GluOC4-5) 0.1 mg $350.00
 

 

Recommended Products
Cat. # Product Package Size Price License
MK111 Gla-Type Osteocalcin (Gla-OC) EIA Kit 96 Assays $699.00  
MK118 Undercarboxylated Osteocalcin (Glu-OC) EIA Kit 96 Rxns $699.00 License Statements
M041 Monoclonal Anti-Bovine Osteocalcin (Clone OC4-30) 0.1 mg $350.00  
 

 


Clontech is a Takara Bio Company © 2012 Privacy Policy | Terms & Conditions | TRUSTe