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Products >  Cell_Biology >  Bone_Research >  Antibodies >  Osteocalcin-Bovine_Monoclonal_Osteocalcin

Osteocalcin Antibody | Monoclonal Anti-Bovine Osteocalcin (Clone OC4-30, OCG2, OCG3 and OCG4)

Monoclonal Anti-Bovine Osteocalcin Antibody (Clones OC4-30,OCG2, OCG3 and OCG4) is raised against bovine osteocalcin and recognizes both bovine and human osteocalcins. Clone OC4-30 is specific for position 17 gamma-carboxylated osteocalcin and does not recognize decarboxylated osteocalcin. Osteocalcin epitopes used to generate Clones OCG2, OCG3 and OCG4 are amino acids 45-49, amino acids 21-31 and amino acids 4-9, respectively. This collection of osteocalcin antibody clones facilitates osteocalcin immunohistochemistry studies which can also be performed on paraffin-embedded tissues (as described in the Resources). In addition to cross-reactivity with bovine and human osteocalcin, clones OCG4-30, OCG3 and OCG4 are also capable of recognizing osteocalcin in several other species as listed. For species-specific osteocalcin detection, Takara Bio offers additional osteocalcin antibody products. 
At-A-Glance Documents Images & Data Resources

Applications

  • All clones can be used for Western blot under reducing or non-reducing conditions.
  • Clones OCG4-30, OCG2 and OCG4 can be used for osteocalcin immunohistochemistry on frozen and paraffin-embedded tissue sections.
  • Clone OCG3 can also be used for osteocalcin immunohistochemistry on frozen tissue sections. Is has weaker reactivity on paraffin-embedded tissue sections than the other clones.

Source

CloneCat.#ImmunogenImmunized hostSubclassAntibody titer
OC4-30M041bovine osteocalcinBALB/c mouseIgG2a1:3,000
OCG2M042bovine osteocalcinBALB/c mouseIgG11:4,000
OCG3M043bovine osteocalcinBALB/c mouseIgG31:1,000
OCG4M044bovine osteocalcinBALB/c mouseIgG11:4,000

Storage

Lyophilized antibody is stable at 4°C for 2 years. The reconstituted solution can be stored at 4°C for 6 months and at –20°C for up to 1 year.

Notes

For research use only. Not for use in diagnostic or therapeutic procedures.

Form

Lyophilized

Reconstitution

Dissolve the lyophilized antibody in 50 µl of Reconstitution Solution. (Final concentration: 2.0 mg/mL)

References

  1. Hauschka, P.U., Lian, J.B., Gallop, P.M. (1975) Proc. Natl. Acad. Sci. U.S.A. 72, 3925.
  2. Engvall, E, and Perlmann, P. (1972) J. Immunol. 109, 129.
  3. Kayama, N., Ohara, K., Yokota, H., Kurome, T., Katayama, M., Hino, F., Kato, I. and Akai, T. (1991) J. Immunol. Methods 139, 17-23.
  4. Vergnaud, P. et al. (1997) J. Clin. Endocrinol. Metab., 82, 719-724.

Specificity

CloneEpitopeReacts withDoes not react with
OC4-30epitope including gamma-carboxylated residue at position 17 (does not react with heat decarboxylated osteocalcin)bovine, human, rat, rabbit, porcine, dog, goat, sheep, chicken, mouse
OCG2residue 45-49bovine, humanmouse, rat
OCG3residue 21-31bovine, human, rat, rabbit, porcine, goat, sheepmouse
OCG4residue 4-9bovine, human, rabbit, porcine, dog, goat, sheep, chickenmouse, rat


 
 
Products
Cat. # Product Package Size Price # of Units Select
M041 Monoclonal Anti-Bovine Osteocalcin (Clone OC4-30) 0.1 mg $357.00
M042 Monoclonal Anti-Bovine Osteocalcin (Clone OCG2) 0.1 mg $357.00
M043 Monoclonal Anti-Bovine Osteocalcin (Clone OCG3) 0.1 mg $357.00
M044 Monoclonal Anti-Bovine Osteocalcin (Clone OCG4) 0.1 mg $357.00
 

 


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