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Products >  Cell_Biology >  Bone_Research >  Antibodies >  Osteocalcin-Bovine_Monoclonal_Osteocalcin

Osteocalcin (Bovine), Monoclonal

These clones were raised against bovine osteocalcin, and equally recognize both the bovine and the human osteocalcins. Clone OC4-30 is specific for gamma-carboxylated osteocalcin.

At-A-Glance Documents Images & Data Resources

Applications

  • Western Blotting analysis under reducing or non-reducing conditions
  • Clones OCG4-30, OCG2, OCG4 can be used for histology on frozen tissue and paraffin embedded tissue sections
  • Clone OCG3 can also be used for histology on frozen tissue sections, and has weaker reactivity for paraffin embedded tissue sections than the other clones

Source

CloneCat.#ImmunogenImmunized hostSubclassAntibody titer
OC4-30M041bovine osteocalcinBALB/c mouseIgG2a1:3,000
OCG2M042bovine osteocalcinBALB/c mouseIgG11:4,000
OCG3M043bovine osteocalcinBALB/c mouseIgG31:1,000
OCG4M044bovine osteocalcinBALB/c mouseIgG11:4,000

Storage

Lyophilized antibody is stable at 4°C for 2 years. The reconstituted solution can be stored at 4°C for 6 months, and at –20°C for 1 year.

Notes

For research use only. Not for use in diagnostic or therapeutic procedures.

Form

Lyophilized

Reconstitution

Dissolve the lyophilized antibody in 50 µL of Reconstitution Solution. (final concentration: 2.0 mg/mL)

References

  1. Hauschka, P.U., Lian, J.B., Gallop, P.M. (1975) Proc. Natl. Acad. Sci. U.S.A. 72, 3925.
  2. Engvall, E, and Perlmann, P. (1972) J. Immunol. 109, 129.
  3. Kayama, N., Ohara, K., Yokota, H., Kurome, T., Katayama, M., Hino, F., Kato, I. and Akai, T. (1991) J. Immunol. Methods 139, 17-23.
  4. Vergnaud, P. et al. (1997) J. Clin. Endocrinol. Metab., 82, 719-724.

Specificity

CloneEpitopeReacts withDoes not react with
OC4-30epitope including gamma-carboxylated residue at position 17 (does not react with heat decarboxylated osteocalcin)bovine, human, rat, rabbit, porcine, dog, goat, sheep, chickenmouse
OCG2residue 45-49bovine, humanmouse, rat
OCG3residue 21-31bovine, human, rat, rabbit, porcine, goat, sheepmouse
OCG4residue 4-9bovine, human, rabbit, porcine, dog, goat, sheep, chickenmouse, rat




 
 
Products
Cat. # Product Package Size Price
M041 Monoclonal Anti-Bovine Osteocalcin (Clone OC4-30) 0.1 mg $350.00
M042 Monoclonal Anti-Bovine Osteocalcin (Clone OCG2) 0.1 mg $350.00
M043 Monoclonal Anti-Bovine Osteocalcin (Clone OCG3) 0.1 mg $350.00
M044 Monoclonal Anti-Bovine Osteocalcin (Clone OCG4) 0.1 mg $350.00
 

 



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