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Products >  Cell_Biology >  Bone_Research >  Antibodies >  Collagen_type_2-Rat_Monoclonal

Rat Collagen Type 2 Monoclonal Antibody: Type-II Collagen (COL2AI)

Type-II Collagen (COL2AI), also referred to as Type 2 Collagen, is a major constitutent of hyaline and elastic cartilage protein. The Rat Collagen Type 2 Monoclonal Antibody specifically recognizes type II collagen in rat, a common research model organism. It is validated for immunoblotting and immunocytochemistry applications.

 

At-A-Glance Documents Images & Data Resources

Applications

  • Western blot (using reducing/non-reducing conditions)
  • Immunocytochemical applications (e.g., immunofluorescence microscopy)

Source

  • Clone: Col II 2B-11F
  • Antigen: Rat type II collagen
  • Immunized host: C57BL/6 mouse
  • Subclass: IgG2b
  • Working concentration: 5-10 µg/ µl for Western blot (colorimetric) or immunocytochemistry

Storage

Store lyophilized antibody at 4°C. Reconstitute in 50 µl distilled water to create a 2.0 mg/mL stock solution. Store aliquots of stock solution at -20°C for up to 1 year or at 4°C for up to 6 months. Avoid repeated freeze-thaw. Do not store diluted antibody.

Properties

Form: Lyophilized

  • Stock solution: Dissolve lyophilized antibody in 50 µl distilled water to create a 2.0 mg/mL stock solution. If storing at 4 °C, add 0.1% NaN3.
  • Recommended dilution solution: For working solutions, dilute in 10 mM PBS (pH 7.4) and 1.0% BSA.

Specificity

  • Rat collagen type II.
  • Does not cross-react with rat collagen type I or III or mouse collagen type II.
  • Weak reactivity with bovine collagen type II.



 
 
Products
Cat. # Product Package Size Price # of Units Select
M192 Monoclonal Anti-Rat Collagen type II (Clone Col II 2B-11F) 0.1 mg $357.00
 

 



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