The ApopLadder Ex Kit selectively extracts the small, fragmented DNA from apoptotic cells and minimizes chromatin contamination. This method supports highly sensitive detection and quantitation of fragmented DNA. Detection of fragmented DNA following isolation can be observed through gel electrophoresis and can be quantitated by fluorescent staining with SYBR Green I (Molecular Probes, Inc). The ApopLadder Ex Kit contains the necessary reagents for cell lysis, enzymatic degradation of protein and RNA, precipitation of the DNA fragments, and a 6X loading dye for gel electrophoresis.
Apoptosis is associated with the fragmentation of chromosomal DNA into approximately 180 bp nucleosome fragments.
