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Products >  Cell_Biology >  Alzheimers_Disease >  ELISA_Kits >  Human_sAPP_ELISA

Human sAPP ELISA Kits

The accumulation of amyloid beta protein in the cerebral cortex in Alzheimer's disease (AD) is only one of the outcomes of proteolytic cleavage of amyloid precursor protein (APP). More than 50 different mutations in the APP gene can cause early-onset Alzheimer disease. Cleavage by beta- and gamma-secretase (amyloidogenic pathway) leads to production of amyloid-beta and soluble APP-beta, while cleavage by alpha-secretase (nonamyloidogenic pathway) results in a soluble form of APP, sAPP-alpha. Several mutations in the SAPP gene can give rise to variants of both SAPP-alpha and sAPP-beta.

These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to human sAPP variant proteins; one is precoated on the ELISA plate and the other is HRP-conjugated. These kits can be used to measure human sAPP variants in serum, EDTA plasma, cerebrospinal fluids, or cell culture supernatant.

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Features

  • Capable of measuring human sAPP variants
  • Sensitivity of 0.06 ng/mL (Cat.# 27731); 0.05 ng/mL (Cat.# 27732); 0.09 ng/mL (Cat.# 27734)

Applications

  • Measuring human sAPP variants in serum, EDTA plasma, cerebrospinal fluids, or cell culture supernatant

Components

Precoated plate: Anti-Human sAPP IgG Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Human sAPP IgG
Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant human sAPP variant protein0.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 0.39–25 ng/mL (Cat.# 27731); 0.78–50 ng/mL (Cat.# 27732, 27734)
  • Assay Sensitivity: 0.06 ng/mL (Cat.# 27731); 0.05 ng/mL (Cat.# 27732); 0.09 ng/mL (Cat.# 27734)
  • Assay duration: Overnight
  • Specificity: Human sAPP-alpha, sAPP-beta-wt, sAPP-beta-Swedish (Cat.# 27731); human sAPP-beta-wt, low cross-reactivity with sAPP-beta-wt/sAPP-beta-Swedish (Cat.# 27732); human sAPP-alpha (Cat.# 27734)
  • Species cross-reactivity: Mouse, rat
  • Test specimen type: Human serum, EDTA plasma, cerebrospinal fluids, or cell culture supernatant
  • Sample volume per well: 100 µL

 
Products
Offers Cat. # Product Contents Size Price Units Select
27731A Human sAPP, Total (highly sensitive) Assay Kit - IBL 96-Well $606.00 *
27732A Human sAPP beta-w (highly sensitive) Assay Kit - IBL 96-Well $606.00 *
27734A Human sAPP alpha (highly sensitive) Assay Kit - IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 

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