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Products >  Cell_Biology >  Alzheimers_Disease >  ELISA_Kits >  Human_Amyloid_beta_ELISA

Amyloid-beta ELISA

The Amyloid-beta ELISA is a solid-phase sandwich ELISA that uses two highly specific antibodies to human amyloid-beta variants; one antibody is precoated onto the ELISA plate and the other is HRP-conjugated. Takara's Amyloid-beta ELISA can be used to measure human amyloid-beta protein variants in EDTA-plasma, cerebrospinal fluid, serum, cell culture media or brain extract.

The accumulation of amyloid-beta protein in the cerebral cortex is responsible for plaque formation and is thought to result in Alzheimer's disease (AD). Amyloid-beta is produced by the proteolytic cleavage of amyloid precursor protein (APP) and is mediated by the enzymes beta- and gamma-secretase. This cleavage reaction produces a number of peptides ranging from 36 to 43 amino acids in length. The Human Amyloid-beta ELISA can detect all amyloid-beta variants that are cleaved at the C-terminal side; however, it cannot detect variants fewer than 16 amino acids in length.


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Features

  • Capable of measuring human amyloid-beta protein variants
  • Sensitive to 3.55 pg/mL

Applications

  • Measurement of human amyloid-beta protein variants in EDTA-plasma, cerebrospinal fluid, serum, cell culture media or brain extract

Components

The Human Amyloid beta (1-x) Assay Kit (Cat.# 27729A) contains the following components:

Precoated plate: Anti-Human Amyloid-Beta (N) (82E1) Mouse
IgG MoAb Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP-conjugated Anti-Human Amyloid-
Beta(11-28) Mouse IgG MoAb Affinity-Purified
0.4 mL x 1
Standard: Human Amyloid-Beta(1-40)1.0 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 7.81–500 pg/mL
  • Assay sensitivity: 0.09 ng/mL
  • Assay duration: Overnight
  • Specificity: Human amyloid-beta C-terminal deletions; shows ≤0.1 cross-reactivity against N-terminal deletions or C-terminal deletions fewer than 16 amino acids
  • Species cross-reactivity: Does not cross-react with mouse or rat amyloid-beta variants
  • Test specimen type: EDTA-plasma, cerebrospinal fluid, serum, cell culture media or brain extract
  • Sample volume per well: 100 µL

 
Products
Offers Cat. # Product Contents Size Price Units Select
27729A Human Amyloid beta (1-x) Assay Kit - IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 

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